草铵膦铵盐的高效液相色谱测定

Determination of Glufosinate-ammonium by HPLC

建立了用高效液相色谱分析草铵膦铵盐的方法。使用NucleosioSB离子交换柱,柱温室温;以0.1mol/L的磷酸二氢钾水溶液为流动相,流速1.0mL/min;紫外检测器,检测波长195nm,外标法对草铵膦铵盐的有效成分进行定量分析。结果表明,草铵膦的线性范围为0.254～2.496g/L,相关系数为0.99914,13.5%草铵膦水剂的标准偏差为0.152,变异系数为1.137%,草铵膦原药的标准偏差为0.2013,变异系数为0.2113%,平均回收率:13.5%草铵膦水剂为100.5%,95%草铵膦原药为99.5%。此方法可用于原药和制剂的分析检测。

A method for rapid determination of glufosinate-ammonium by HPLC was established. The operating conditions of quantitative determination glufosinate-ammonium HPLC with external standard method were Nucleosio SB ion exchange as colomun at ambient temperature, 0.1 mol/L phosphate buffer （KH2PO,） as mobile phase with the flow rate of 1.0 mL/min and UV detector wavelength at 195 nm. The linear rang was 0.254-2.496 g/L, linear correlation coefficient was 0.99914. The standard deviations were 0.152 for 13.5% water formulation and 0.2013 for technology, average recoveries were 100.5% for 13.5% water formulation and 99.5% for technology. This method could applied to the determination of the formulations and technology.