肾康注射液对单侧输尿管梗阻大鼠模型肾间质转化生长因子-β_1和骨形成蛋白-7表达的影响

Effect of Shenkang Injection on Transforming Growth Factor-β_1 and Bone Morphogenetic Protein-7 in Rats with Unilateral Ureteral Obstruction

目的研究肾康注射液对单侧输尿管梗阻(UUO)大鼠模型肾间质转化生长因子-β1(TGF-β1)和骨形成蛋白-7(BMP-7)表达的影响。方法将SD雌性大鼠54只随机分为正常对照组、UUO模型组、肾康治疗组,肾康治疗组用肾康注射液灌胃,分别在手术后第5,10,15天处死大鼠,在光镜下观察肾组织病理改变,应用免疫组织化学方法检测肾小管间质中TGF-β1和BMP-7表达。结果与正常对照组相比,UUO模型组大鼠肾脏TGF-β1表达明显升高,BMP-7表达明显减少,但在予肾康注射液治疗后能显著下调UUO模型大鼠TGF-β1表达,上调BMP-7表达。结论肾康注射液可减轻肾小管间质纤维化,延缓慢性肾功能衰竭进展。

Objectlve To study the effect of Shenkang Injection on transforming growth factor-β1（TGF-β1） and the bone morphogenetic protein-7（BMP-7） in rats with unilateral ureteral obstruction. Methods To divide 54 SD female rats into 3 groups randomly： control group, model group, and Shenkang group was given. Shenkang group Shenkang Injection by gavage. Six rats of each group were sacrificed on 5, 10, 15 d respectively after operation. To observe the pathological changes of renal tissue and to use immunohistochemistry for checking the expression levels of TGF-β1 and BMP- 7 in renal tubular and interstitial. Results Compared with normal group, the levels of TGF-β1 in model group was signifcantly increased and BMP- 7 was significantly reduced. TGF-β1 of Shengkang group was remarkably reduced and BMP- 7 was increased. Conclusion Shenkang Injection can prevent the development of renal tubulointerstital fibrosis and retard the progress of chronic renal failure.