TNFR Ⅱ基因-196M/R多态性与乳腺癌易感性的相关性

Association between TNFR Ⅱ gene polymorphisms and breast cancer

目的探讨TNF-α受体Ⅱ(TNFR Ⅱ)基因第6外显子-196M/R多态性与中国北方女性乳腺癌发病的相关性。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测TNFR Ⅱ基因-196M/R多态性,分析212例乳腺癌患者(实验组),218例乳腺良性病变患者(良性组)和220例健康对照者(对照组)的TNF-α受体Ⅱ(TNFR Ⅱ)基因多态性与乳腺癌易感性的关系。结果(1)650例研究对象中存在TNFR Ⅱ基因-196M/R多态性。(2)-196M/R位点的TG+GG基因型及G等位基因频率在良性病变组与对照组间差异无统计学意义,而在乳腺癌组则显著高于良性病变组和对照组(均P(0.05)。结论TNFR Ⅱ基因-196M/R在乳腺癌组和对照组存在多态性,第6外显子-196M/R位点T/G多态性可能为乳腺癌发病的易感基因位点,G等位基因可能为中国北方女性乳腺癌发病的易感基因。

Objective To explore the relationship between TNF-α receptor Ⅱ（TNFR II）gene - 196M/R polymorphisms and breast cancer, Methods TNFR Ⅱ gene polymorphisms at position - 196 were determined by polymerase chain reaction- restriction fragment length polymorphism （PCR-RFLP） method in 212 female patients with breast cancer,218 female patients with breast benign diseases and 220 female healthy controls. Results （1） There were TNFR II gene - 196 M/R polymorphisms in the 640 cases, （2） There was no statistic difference in the frequencies of TG ＋ GG genotype and allele G between benign and control groups. The frequencies of TG ＋ GG genotype in the study group were significantly higher than those in the benign and control groups （P 〈 0.05 ）. Conclusion There were TNFR II gene - 196 M/R polymorphisms in breast cancer and control groups. TNFR II gene - 196 G allele may be the predisposing gene and the relative risk of breast cancer.