小型猪火器伤后血浆和组织中白细胞介素6表达的意义

Expressions of interleukin-6 in blood and tissue after firearm injuries in pigs

目的通过检测火器伤后白细胞介素6(interleukin-6,IL-6)在血浆和组织中的表达,探讨创伤时IL-6的变化规律及其与创伤严重程度的关系。方法制作火器伤模型,24只小型猪随机分为对照组(C组,不致伤)、颅骨切线伤组(H组)、双侧股骨骨折组(L组)和多发伤组(M组),每组6只。采用7TD1(IL-6依赖的杂交瘤细胞株)生物法测定外周血和门静脉血IL-6活性。PCR方法测定各组动物肺、肝组织IL-6mRNA的表达情况。结果C组各项指标无明显变化;各创伤组伤后6h开始体循环血浆IL-6明显增高。创伤后门静脉血浆IL-6水平变化升高更明显,肝、肺组织IL-6mRNA的表达明显高于对照组,M组伤后血中IL-6水平和肺、肝组织IL-6mRNA的表达又较H、L两组更高。结论创伤后IL-6在血中和组织中的表达总体上是升高的,表达水平的高低与创伤的程度是一致的。

Objective To discuss the mechanism of IL-6 changes after firearm injuries and the rela- tionship between the changes and the severity of injuries. Methods 5.8 mm bullets were used to make various injuries. 24 health pigs were divided into 4 groups. Control group （Group C, n=6） was subjected to catherization only,group H（n=6） was subjected to tangental fracture of parietal bone. Comminuted fractures group （Group L, n=6） was subjected to tangental fracture of parietal bone and comminuted fractures of bilateral femora. Multiple trauma group （group M, n=6） was subjected to the injuries of Group H ＋ L .The bioativities of IL-6 in portal blood and systemic circulation were detected with biological methods. The expression of IL-6 mRNA was assayed in lungs and livers before and after trauma. Results The plasma concentration of IL-6 in systemic circulation was increased obviously in 6 hours after trauma. The bioativities in portal blood increased more significantly than that in systemic circulation. The enhanced expression of IL-6 mRNA in lungs and livers were found after trauma. No significant changes were found in group C. Conclusion The expression of IL-6 in circulation or tissue are increased obviously after firearm injuries. The concentration of IL-6 may be associated with the injury severity.