摘要
目的:探讨供体特异输注(donor-specific transfusion,DST)及不同剂量FK506对同种异体小鼠心脏移植的影响。方法:应用显微外科技术制作颈部移植心脏急性排斥反应小鼠模型,将移植受体小鼠分为4组:对照组(单纯移植组,未加DST),DST+移植组,DST+移植+FK506(2 mg/(kg·d))组,DST+移植+FK506(0.3 mg/(kg·d))组,比较各组移植心脏生存时间,心肌病理改变及外周血血清细胞因子水平:血清IL-2,IL-4,IL-10和IFN-γ水平。结果:术前1天应用DST与连续应用较小剂量FK506可显著延长移植物存活。术后第七天病理检查发现联合应用DST和FK506的两组移植物急性免疫排斥反应明显比其他两组减轻。血清中IL-2和IFN-γ水平在联合应用DST和FK506的两组明显低于其它两组,IL-4和IL-10水平在联合应用DST和FK506的两组高于对照组和DST+移植组。结论:术前DST及持续应用较小剂量FK506可有效抑制同种异体小鼠颈部心脏移植术后急性排斥反应,显著延长移植物的生存时间。
Objective: To investigate the effects of donor-specific transfusion (DST) and different doses of FK506 on the survival of homologous cardiac transplantation in mice. Methods: Male BALB/C (H-2d) mouse hearts were transplanted to male C57BL/6 (H-2b) mice using Chen's model. Mice were randomly divided into four groups: the control group, the DST group, the DST/FK506 (2 mg/(kg·d) ) group and the DST/FK506 (0.3 mg/(kg·d) ) group. Their duration of survival, pathological changes in cardiac muscle, and serum IL-2,IL-4, IL-10 and INF-γ levels were compared. Results: ① DST used at the day before operation and FK506 used sequently from the beginning to the end, the longer cardiac allograft survival was observed in the DST/FK506 (2 mg/ (kg·d)) group and the DST/FK506 (0.3 mg/(kg·d) ) group than those in the control group (P〈0.01 ). Among DST/FK506 groups, the low dose of FK506 as aid to DST was the most effective (group 4 vs group 3, P〈0.05 ). ② Pathological changes: Seven days after transplantation, severe rejections were found in mice in group 1 and group 2 with histological grading above 3. Mild rejections were found in mice treated with FK506 and DST in group 3 and group 4 with histological grading no more than 2. ③ Serum cytokine levels: IL-2 levels in group 3 and group 4 were higher compared with that in group 1 and group 2. IL-4 levels in group 3 and group 4 were lower compared with that in group 1 and group 2. IL-10 levels in group 3 and group 4 were lower compared with that in group 1 and group 2. INF-γ levels in group 3 and group 4 were higher compared with that in group 1 and group 2. Conclusion: DST and low dose FK506 can effectively suppress acute rejection after cardiac allo-transplantation in mice, and prolong the murine cardiac allograft survival.
出处
《现代生物医学进展》
CAS
2008年第7期1229-1232,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(30371400)