期刊文献+

供体特异输注及FK506对同种异体小鼠心脏移植急性排斥反应的影响

Effects of Donor-Specific Transfusion and FK506 on Cardiac Allo-transplantation in Mice during Acute Rejection
下载PDF
导出
摘要 目的:探讨供体特异输注(donor-specific transfusion,DST)及不同剂量FK506对同种异体小鼠心脏移植的影响。方法:应用显微外科技术制作颈部移植心脏急性排斥反应小鼠模型,将移植受体小鼠分为4组:对照组(单纯移植组,未加DST),DST+移植组,DST+移植+FK506(2 mg/(kg·d))组,DST+移植+FK506(0.3 mg/(kg·d))组,比较各组移植心脏生存时间,心肌病理改变及外周血血清细胞因子水平:血清IL-2,IL-4,IL-10和IFN-γ水平。结果:术前1天应用DST与连续应用较小剂量FK506可显著延长移植物存活。术后第七天病理检查发现联合应用DST和FK506的两组移植物急性免疫排斥反应明显比其他两组减轻。血清中IL-2和IFN-γ水平在联合应用DST和FK506的两组明显低于其它两组,IL-4和IL-10水平在联合应用DST和FK506的两组高于对照组和DST+移植组。结论:术前DST及持续应用较小剂量FK506可有效抑制同种异体小鼠颈部心脏移植术后急性排斥反应,显著延长移植物的生存时间。 Objective: To investigate the effects of donor-specific transfusion (DST) and different doses of FK506 on the survival of homologous cardiac transplantation in mice. Methods: Male BALB/C (H-2d) mouse hearts were transplanted to male C57BL/6 (H-2b) mice using Chen's model. Mice were randomly divided into four groups: the control group, the DST group, the DST/FK506 (2 mg/(kg·d) ) group and the DST/FK506 (0.3 mg/(kg·d) ) group. Their duration of survival, pathological changes in cardiac muscle, and serum IL-2,IL-4, IL-10 and INF-γ levels were compared. Results: ① DST used at the day before operation and FK506 used sequently from the beginning to the end, the longer cardiac allograft survival was observed in the DST/FK506 (2 mg/ (kg·d)) group and the DST/FK506 (0.3 mg/(kg·d) ) group than those in the control group (P〈0.01 ). Among DST/FK506 groups, the low dose of FK506 as aid to DST was the most effective (group 4 vs group 3, P〈0.05 ). ② Pathological changes: Seven days after transplantation, severe rejections were found in mice in group 1 and group 2 with histological grading above 3. Mild rejections were found in mice treated with FK506 and DST in group 3 and group 4 with histological grading no more than 2. ③ Serum cytokine levels: IL-2 levels in group 3 and group 4 were higher compared with that in group 1 and group 2. IL-4 levels in group 3 and group 4 were lower compared with that in group 1 and group 2. IL-10 levels in group 3 and group 4 were lower compared with that in group 1 and group 2. INF-γ levels in group 3 and group 4 were higher compared with that in group 1 and group 2. Conclusion: DST and low dose FK506 can effectively suppress acute rejection after cardiac allo-transplantation in mice, and prolong the murine cardiac allograft survival.
出处 《现代生物医学进展》 CAS 2008年第7期1229-1232,共4页 Progress in Modern Biomedicine
基金 国家自然科学基金项目(30371400)
关键词 供体特异输注 FK506 同种异体颈部心脏移植 小鼠 Donor-specific transfusion FK506 Cardic allo-transplantation Mouse
  • 引文网络
  • 相关文献

参考文献15

  • 1Siemionow M,Agaoglu G.Role of blood transfusion in transplantation:a review[J].Reconstr Microsurg,2005,21(8):555-563
  • 2Chen Z.A new technique of cervical heterotopic heart transplantation in mice[J].Transplantation,1991,52(6):1099-1101
  • 3The International Society of Heart Transplantation.A working formulation for the standardization of nomenclature heart and lung rejection[J].Heart Transplant,1990,9:587
  • 4Okazaki H,Sato T,Miura S,et al.Donor-specific blood transfusion in living-related renal transplantation:fourteen-year experience[J].Transplant Proc,1997,29:200-202
  • 5Miura S,Okazaki H,Satoh T,et al.Long-term follow-up of living donor renal transplant recipients sensitized after donor specific blood transfusion[J].Transplant Proc,2001,33:1221-1223
  • 6Liang J,Yamaguchi Y,Matsuda T,et al.Posttransplant infusion of donor-specific blood induces immunological unresponsiveness in rat hepatic allografts[J].Transplantation,2000,70:1363-1371
  • 7Sykes M.Mixed Chimerism and Transplant Tolerance[J].Immunity,2001,14:417-424
  • 8Takeuchi T,Lowry RP,Konicczny B.Heart allografts in murine systems.The differential activation of Th2-like effector cells in peripheral tolerance[J].Transplantation,1992,53:1281-1294
  • 9Potter D,Garovoy M,Hopper S,et al.Effect of donor specific transfusion on renal transplantation[J].Pediatrics,1985,76:402-405
  • 10Hamawy MM.Molecular actions of calcineurin inhibitors[J].Drug News Perspect,2003,16(5):277-282
;
使用帮助 返回顶部