一株产碱性蛋白酶的嗜碱芽孢杆菌的分离和鉴定

Isolation and Identification of Bacillus Alcalophilus with Alkaline Protease Activity

从天津塘沽盐碱土壤中分离一株产碱性蛋白酶的嗜碱芽孢杆菌HAP，并对其进行了表型分类和16S rDNA序列分析。结果表明，HAP是一株革兰氏阳性芽孢杆菌，菌体大小（0．7—0．9）μm×（2-3）μm，该菌可利用木糖、葡萄糖、阿拉伯糖、甘露醇，发酵型糖代谢产酸；可以水解酪素、淀粉，但不能水解酪氨酸；能够利用柠檬酸盐；其酶活力为1．22×10^4U／mL。结合系统发育学分析将HAP鉴定为嗜碱芽孢杆菌（Bacillus alcalophilus）。

An isolate from alkali soil of Tanggu, Tianjin with alkaline protease activity was identified by phenotypic classification and phylogenetic analysis. The results show that the strain HAP is a gram-postive bacillus with about （0.7-0.9） μm×（2-3） μm width×length. Many kinds of carbon sources and nitrogen sources could be utilized in the medium. Physiological and biochemical test showed that xylose, glucose, arabinose and mannose can be utilized by the strain as fermentable sugar for acid production. The strain could growth well in the substrates of casin and starch, and however, not in the substrate of tyrosine. It could utilize citrate, too. A sequence alignment of 16s rDNA of the strain HAP was performed and the phylogenetic tree was constructed. The strain was identified as Bacillus alealophilus. The protease activity of strain HAP is 1.22×10^4 U/mL.