Rho激酶对结直肠癌细胞体外侵袭转移作用的研究

Effects of Rho kinase on invasion and immigration of in vitro colorectal cancer cell

目的:观察Rho激酶对结直肠癌SW480细胞黏附、运动、迁移和侵袭能力的影响。方法:SW480细胞用Rho激酶抑制剂Y-27632处理后,分别用细胞运动实验,黏附实验和迁移、侵袭实验分析对肿瘤细胞运动、黏附、迁移和侵袭能力的影响。结果:25μmol/L以上的浓度的Y-27632作用于SW480细胞后,细胞的运动能力、迁移能力明显下降(p<0.05),细胞黏附于纤维粘连蛋白的能力也降低(p<0.05);而且细胞穿过人工基底膜的能力明显降低(p<0.01)。结论:Rho激酶参与结直肠癌细胞体外的侵袭转移作用,Rho激酶抑制剂有望成为有效的抗恶性肿瘤转移的药物。

Objective： To investigate the effects of Rho kinase on cell adherence, movement, migration and invasion of colorectal cancer cell. Method： After treatment of SW480 cell with Rho kinase inhibitor Y-27632, Cell biological behaviors such as adherence, movement, migration and invasion were observed. Results： After the treatment of Y-27632 whose concentration is higher than 25 μmol/L, the ability of SW480 cells, including motility, migration, attachment to fibronectin and crossing the reconstituted basement membrane, which were decreased significantly （p〈0.05）. Conclusion： Rho kinase is involved in the immigration and invasion of colorectal cancer cell. The selective inhibitor of Rho kinase might be a candidate drug for tumor therapy.