刺葡萄籽油功效成分及其动物学药理作用研究

Study on Functional Compositions and Pharmacological Effect ofVitis Davidii Foёx

目的:研究刺葡萄籽油中的功效成分及其动物学药理作用。方法:使用GC/MS对甲酯化和未经甲酯化处理的刺葡萄籽油进行分析,研究籽油中的功效成分,并以小白鼠为研究对象进行刺葡萄籽油的喂食。结果:超临界CO_2萃取的刺葡萄籽油的GC/MS分析结果表明,刺葡萄籽油成分以脂肪酸为主,其中不饱和脂肪酸含量占87%,不饱和脂肪酸主要为亚油酸,其含量达82.32%,高于已有报道的葡萄籽油中亚油酸含量;未经甲酯化处理的籽油经GC/MS分析发现,刺葡萄籽油中含有一种具有较强生物活性的成分—角鲨烯,而赤霞珠葡萄的GC/MS分析中没有发现,也未见葡萄属植物中含有角鲨烯成分的研究报道。通过动物学药理作用实验发现,刺葡萄籽油不仅能显著降低血清总胆固醇(TC)、甘油三脂(TG)含量,还能够在降低低密度脂蛋白胆固醇(LDL-C)的同时,提高高密度脂蛋白胆回醇(HDL-C)的含量。结论:刺葡萄籽油中含有多种对人体有益的功效成分,值得人们进一步地研究。

Objective： To study functional compositions and pharmacological effect of Vitis davidii Foex. Methods： The functional compositions of methyl esterified and not methyl esterified Vitis davidii Foex seed oil were Analysed respectively by GC/MS and the model of Vitis davidii Foex. SD rats were fed with seed oil. Results Result of SCF-CO2 extracted from Vitis davidii Foex seed oil by gas chromatography-mass spectrometry （GC/MS） indicated that the oil contained 87% unsaturated fatty acids, which contained 82.32% linoleic acid. It was higher than other reports of grape seed oil. Squalene was found firstly in the grape （ Vitis davidii Foex） seed oil which wasn＇t methyl esterified by GC/MS method. Experiment hyperllpemla model was established with SD rats to observe the change of biochemistry data of plasma lipid. The results showed that rats＇ TC, TG, LDL-C were significantly decreased and HDL-C were increased. Conclusion： Vitis davidii Foex seed oil includes many functional compositions which are beneficial for health, and it is worth for further study.