系统性红斑狼疮患者淋巴细胞共刺激分子异常表达的研究

Investigation of costimulatory molecules of lymphocytes in systemic erythematosus lupus （SLE） patients

目的探讨系统性红斑狼疮（SLE）患者淋巴细胞共刺激分子的表达及意义。方法采用流式细胞术检测SLE患者淋巴细胞CD28、CTLA-4、CD80和CD86的表达，并与对照组比较。结果与健康对照者相比较，SLE患者CD3^＋细胞增加（P〈0．05），CD3^＋CD4^＋细胞降低（P〈0．05），CD3^＋CD8^＋细胞升高（P〈0．05），CD4／CD8比例明显倒置（P〈0．01），CD28表达降低，CTLA-4升高，CD86的表达显著高于正常人（P〈0．01），CD80在SLE患者CD19^＋B细胞上的表达与正常人无异（P〉0．05）。结论T细胞亚群改变及T、B淋巴细胞共刺激分子CD28、CTLA-4和CD86在SLE发病机制中起重要作用。

Objective To investigate the roles of lymphocytes costimulatory molecules in systemic erythematosus lupus （SLE）. Methods CD28, CTLA-4, CD80 and CD86 in lymphocytes in peripheral blood were detected with flow cytometry. Results T lymphocytes subsets changed in patients with SLE. Compared with healthy control, SLE patients have higher percentage of lymphocytes expressing CTLA-4, CD86, lower percentage of lymphocytes expressing CD28 and that of CD80 didn＇ t changed. Conclusion The changes of T lymphocyte subsets and lymphocyte costimulatory molecules CD28, CTLA-4 and CD86 may have roles in the development of SLE.