疏水电荷诱导层析用于抗体纯化的研究进展

The Development of Antibody separation by hydrophobic charge induction chromatography

单克隆抗体、多克隆抗体及抗体片段在科学研究和医药领域中的位置越来越重要，抗体的纯化技术也在不断发展。疏水电荷诱导层析是最近几年发展起来的一项新技术，该技术的基础是一种双模式的配基，这种配基对盐离子的浓度没有依赖。在中性pH条件下，通过疏水相互作用与蛋白结合，在酸性条件下与蛋白分离。由于该技术在抗体纯化方面具有操作简单、费用低、纯度高等优点而被迅速用于抗体的分离和纯化。了解疏水电荷诱导层析的基本原理、发展概况以及在抗体纯化方面的应用很重要。

Monoclonal and polyclonal antibodies play a key role in medicine as well as in analytical biotechnology. The methods for purification of antibodies are developed fast. Hydrophobic charge induction chromatography （HCIC） is a recently developed method for protein separation based on the use of dual-mode ligands. They are designed in such a way so as to combine a molecular interaction supported by a mild hydrophobic association effect in the absence of salts. When pH is changed ,the ligand becomes ionically charged resulting into the desorption of the protein. HCIC shows specific advantages （ such as high degree of purity and recovery） and is used in several labs and manufactures for antibody production. This paper reviewed the basic principle, development and application of HCIC.