不锈钢和氧化钛薄膜刺激内皮细胞释放NO和MCP-1的研究(英文)

Effects of Stainless Steel and Titanium Oxide in Stimulation of Endothelial Cells Releasing NO and MCP-1

目的:探讨不锈钢和氧化钛(Ti-O)薄膜对内皮细胞释放细胞因子的影响,评价生物材料表面的内皮细胞的功能。方法:用非平衡磁控溅射的方法制备Ti-O薄膜,提取人脐静脉内皮细胞(HUVEC)并种植到材料表面进行培养。第1、3、5天提取培养上清液,用NO检测试剂盒和酶联免疫吸附实验的方法进行一氧化氮(NO)和单核细胞趋化蛋白-1(Monocyte Chemotactic Protein 1,MCP-1)的检测,采用环境扫描电子显微镜(SEM)进行细胞形态观察。结果:内皮细胞在Ti-O薄膜表面上生长形态保持较好,其培养上清液NO和MCP-1因子释放量都要略小于不锈钢表面的。结论:Ti-O薄膜具有较好的促进内皮化和组织相容性,有望成为血管支架的新型生物材料。

To investigate the influence of stainless steel （ SS ）and titanium oxide （ Ti-O ）thin films on the cytokine released by endothelial cells. Ti-O films were prepared by unbalanced magnetron sputtering deposition system （UBMS）. Human umbilical vein endothelial cells （HUVECs）were isolated and seeded on SS and Ti-O materials. Nitride oxide （NO）and monocyte chemotactic protein one （MCP-1）concentrations were determined in the supernatants with enzyme linked immunosorbent assay （ELISA）at 1,3 and 5 days after culture, and the morphology of the cells was observed using scanning electron microscopy （ SEM ）. The results showed that the similar amount of HUVECs was presented in SS and Ti-O culture, but HUVEC had better morphology on Ti-O film than SS. The NO and MCP-1 test also showed that HUVECs on Ti-O film had a better bio-function than SS. It is concluded that Ti-O film as a biomaterial may have a better endothelial compatibility, which can be used to enhance the endothelialization.