α-淀粉酶水解魔芋飞粉最佳条件优化的研究

Research in the optimum conditions of hydrolyzing konjac fly powder with α-amylase

本研究采用单因素和正交试验的方法对α-淀粉酶水解魔芋飞粉的酶用量、pH值、温度、[Ca^(2+)]和底物浓度等条件进行优化,结果表明当α-淀粉酶用量为4u/g淀粉、pH为6.0、温度为60℃、[Ca^(2+)]为0.01mol/L和底物浓度为8%时,水解度达20%,为最佳反应条件,研究为进一步利用α-淀粉酶水解魔芋飞粉生产燃料乙醇奠定了基础。

In this paper, the optimal conditions of konjac fly powder hydrolysed by α-amylase were established by single factor tests and orthogonal experiment, including the dosage of enzyme, pH, temperature, the concentration of Ca^2＋ and substrate. The results showed that the optimum conditions were as follows： the dosage of enzyme 4u/g starch, pH 6.0, temperature 60℃, the concentration of Ca^2＋ 0.01 mol/L, the concentration of substratum 8%. Under these optimum conditions, the degree of hydrolysis is 20%. The present study established a foundation for fuel ethanol production by konjac fly powder hydrolysed by α-amylase.