甘氨酸对缺血缺氧心肌细胞能量代谢的影响

Protective effects of Glycine in cardiomyocyte subjected to ischemia and hypoxia

目的:明确甘氨酸对缺血缺氧心肌细胞具有保护作用。方法:建立心肌细胞缺血缺氧模型,施加甘氨酸干预,应用高效液相色谱观察心肌细胞ATP、ADP、AMP含量变化及ATP/ADP比值。结果:模拟缺血缺氧1 h后开始心肌细胞ATP含量较正常组明显降低(P<0.01),而ADP、AMP含量与ATP变化相反,ATP/ADP比值较正常组也明显降低(P<0.01);甘氨酸干预复合缺血缺氧后,心肌细胞ATP含量及ATP/ADP比值较单纯缺血缺氧组显著降低(P<0.01),而ADP、AMP含量明显升高(P<0.01)。结论:甘氨酸可以明显改善缺血缺氧心肌细胞的能量储备,发挥其保护效应。

Objective： To investigate the protective effects of glycine in cardiomyocyte subjected on ischemia and hypoxia. Methods： Cardiomyocytes model were established in vitro. The content ofATP, ADP and AMP were tested by high performance liquid chromatogram （HPLC）. Results： The content of ATP of cardiomyocytes subjected to ischemia and hypoxia were significantly lower （P〈0.01） after 1 h. On the contrary, the eontent of ADP and AMP were significantly higher. The change of ATP/ADP was similar with ATP. However, when cardiomyocytes were subjected to glycine （5 mmol/L） combined with ischemia and hypoxia, the content of ATP and ATP/ADP were increased and the content of ADP and AMP were significantly decreased than those without treatment of glycine （P〈0.01）. Conclusion： Glycine can improve obviously the energy metabolism of cardiomyocytes subjected to ischemia and bring into protection effect.