细菌人工染色体的修饰及其转基因应用研究

Study on Modification Methods of BAC and the Application of BAC in Transgene

细菌人工染色体(Bacterial Artificial Chromosome,BAC)是一种新发展起来的DNA载体系统,它具有容量大、遗传特性稳定、易于操作等优点。广泛的应用于基因组文库构建、基因功能分析等方面。随着基因组测序工程的实施与完成,如何对包含完整基因信息的特定细菌人工染色体进行有目的修饰已成为功能基因组学研究的一个重要环节。BAC载体转基因技术可能成为避开基因打靶获得高效表达的转基因生物的另一途径。本文介绍了BAC作为转基因栽体的几种修饰方法及其在转基因研究上的应用。

As a newly developed vector system, the bacterial artificial chromosome （BAC） has several advantages, including large capacity, stable heritage and easy handling. It is utilized extensively in libraries construction, genome research and analysis of gene function. With the completion of genome-sequencing projects, intentional modification of definite bacterial artificial chromosomes, which carry entire components of most eukaryotic genes, is becoming more important for the subsequent functional genomics studies. BAC vectors may be another approach to obtain transgenic biology with high efficiency and high expression without gene targeting. Some modification methods of BAC and the application of BAC in transgene research were reviewed in present article.