金属螯合亲和层析的应用研究进展

Research Progress of Metal-Chelate Affinity Chromatography

金属螯合亲和层析是近30年来出现的一种新型的分离纯化技术,它以配基简单、吸附量大、分离条件温和、通用性强等特点,逐渐成为分离纯化蛋白质等生物工程产品最有效的技术之一。其在蛋白质、酶和核苷酸纯化以及蛋白质分析等方面具有广泛的应用。而随着研究的进一步深入,其新应用也越来越广泛的被发现。

Metal-Chelate Affinity Chromatography （MCAC） can be used to isolate desired proteins from a mixture basing on their affinity difference. MCAC has become a powerful tool for separation of biotechnological products, such as proteins, amino acids and gene products as its large adsorption capacity, mild separation condition, simple ligands and wide applications. With the development of MCAC, more and more new applications will be discovered.