NaCl胁迫对黄瓜嫁接苗耐盐性的影响

Effects of NaCl Stress on Salt Tolerance of Grafted Cucumber Seedlings

为阐明嫁接对黄瓜耐盐性的影响。对日光温室黄瓜嫁接苗和自根苗在150 mmoL/L的NaCl胁迫下的保护酶活性、渗透调节物质及光合特性进行测定和比较。结果表明,黄瓜嫁接苗叶片中过氧化物酶(POD)、超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)活性以及抗坏血酸(AsA)、叶绿素(Chl.)含量均明显高于自根苗;游离脯氨酸、可溶性蛋白质、可溶性糖含量均明显高于自根苗;净光合速率、气孔导度、胞间CO2浓度均明显高于自根苗;丙二醛(MDA)含量和气孔限制值低于自根苗。以上结果表明,嫁接苗能提高黄瓜的耐盐性。

For clarifying the effect of grafting on cucumber salt tolerance, the activities of protective enzymes, contents of osmotic adjustment substances and photosynthetic characteristics were analysed respectively between grafted and scion-root cucumber seedlings under NaCl stress of 150 mmol/L in sunlight greenhouse. The results showed activities of peroxidase （POD）, superoxide dismutase （SOD）, ascorbatespecific peroxidase （ A PX ）, and content of ascorbic acid （AsA）, chlorophyll in leaves of grafted seedlings were significantly higher than those of scion-root seedlings. The content of free proline, soluble protein, soluble suger in the leaves of grafted seedlings were significantly higher than those of scion-root seedlings. Net photosynthetic rate, stomatal conductance, intercellular CO2 concentration of grafted seedlings were signifi- cantly higher than those of scion-root seedlings. Malondiadehyde（MDA）content and stomata unitation of grafted seedlinds were lower than those of scion-root seedlings. These results indicated that grafted seedlings could, mprove cucumber salt tolerance under NaCl stress.