短日照对金叶女贞茎抗寒性和电阻抗图谱参数的影响

The Influence of Short-day on the Frost Hardiness and Electrical Impedance Spectroscopy Parameters in Stems of Ligustrum vicaryi During Hardening

为探明短日照处理对金叶女贞(Ligustrum vicaryi)茎抗寒性和电阻抗图谱参数的影响,找出抗寒性和电阻抗图谱参数的关系,完善电阻抗图谱法测定植物抗寒性技术,在抗寒锻炼前,对金叶女贞当年生扦插苗进行短日照处理,对照为自然日照长度;然后分别用电阻抗图谱法和电导法估测其抗寒性,并进行比较分析。结果表明:短日照处理能在抗寒锻炼期间使金叶女贞茎的抗寒性提高3℃左右,金叶女贞茎的抗寒性与未冷冻处理茎的弛豫时间τ、冷冻处理后茎的胞外电阻率re、含水量之间均存在较高的相关性。电阻抗图谱法是一种测定植物抗寒性,尤其是估测不经冷冻处理样本抗寒性的可行的方法。

To study the influence of short-day（SD）treatment on the frost hardiness（FH）and the parameters of electrical impedance spectroscopy（EIS）in stems of Ligustrum vicaryi during the hardening,as well as to find the relation between the EIS parameters and the FH, and perfect EIS method for studying FH on plant, the cutting plants of Ligustrurn vicaryi was short-day treated before frost hardening on 6 September, 2006, and the natural photoperiod was used as control. Then the FH was assessed by methods of EIS and conventional electrolyte leakage（EL）, and the FH results between the two methods were compared. The results indicated that the FH of the stem in plants treated with SD was hardier about 3 ℃ than that in control plants during the hardening. The FH had high correlation with relaxation time （ τ ） of stem without controlled freezing, extracellular resistance （re） of stems after controlled freezing tests and the water content of the stems. The EIS is one of the most promising methods for assessing FH, especially without employing a controlled freezing test.