上皮性卵巢癌组织中Seprase的表达及意义

Expression of Seprase mRNA and protein in epithelial ovarian carcinoma and its clinical significance

目的探讨Seprase在上皮性卵巢癌组织中的表达及其与临床病理因素的关系。方法采用显微切割技术对31例新鲜冰冻的卵巢癌组织(实验组)进行处理,获取较纯的卵巢癌细胞,采用RT-PCR和Western blot技术检测Seprase mRNA和蛋白的表达,与9例正常卵巢组织(对照组)作对比。结果实验组全部表达SeprasemRNA,而对照组仅3例(33.33%)表达。卵巢癌组织和正常组织中Seprase mRNA的相对含量分别1.13±0.27和0.47±0.13,两者相比,P<0.05。实验组26例(83.87%)表达Seprase蛋白,而对照组仅2例(22.22%)表达。卵巢癌组织和正常组织中Seprase蛋白的相对表达量分别为1.29±0.37和0.51±0.19,两者相比,P<0.05。Seprase蛋白的表达与卵巢癌FIGO分期、组织学分级有关(P<0.05)。Seprase mRNA表达与卵巢癌组织学分级有关(P<0.05),而与患者年龄、肿瘤大小、FIGO分期、淋巴结转移和组织学类型无关(P>0.05)。结论Seprase参与了卵巢癌的发生发展,可能成为卵巢癌的诊断指标及基因治疗靶点。

Objective To explore the expression of Seprase mRNA and protein in fresh ovarian carcinoma tissues, as well as evaluate its association with clinicopathological parameters. Methods Microselection was performed in 31 frozen epithelial ovarian carcinoma tissues to capture cancer cells. Then the expression pattern of Seprase mRNA and protein was examined by RT-PCR and western blot. 9 normal ovarian tissues were detected as control. Results All of 31 specimens were positive for Seprase mRNA by RT-PCR while 3 in counterpart. The level of Seprase mRNA were 1.13±0. 27and 0. 47 ± 0. 13 （P 〈 0. 05）in ovarian carcinoma tissues and its counterpart, respectively. Seprase protein expression was observed in 26 specimens from epithelial ovarian carcinoma while only 2 in control. The levels of protein were 1.29 ± 0. 37 and 0.51 ± 0. 19（ P 〈 0. 05）in epithelial ovarian carcinoma tissues and normal controls, respectively. Increased Seprase protein expression was associated with patients＂ FIGO stage and grade, while mRNA expression was associated with patients＂ grade. Conclusions Our results imply that Seprase be involved in the progression of epithelial ovarian cancer, indicating that Seprase may be a potential predictive marker for epithelial ovarian carcinoma and a potential target for gene therapy.