DOX-PBCA-NP的制备及其对脑胶质瘤SHG44细胞的抑制作用观察

Preparation of doxorubicin-polybutylcyanoacrylate nanoparticles and study on its inhibition effect with SHG44 cells

目的制备阿霉素(DOX)-聚氢基丙烯酸丁酯(PBCA)-纳米粒(NP),观察其在体外对脑胶质瘤SHG44细胞的抑制作用。方法采用乳化聚合法制备DOX-PBCA-NP,透射电镜观察其形态,紫外分光光度法测定其载药量和包封率。将SHG44细胞加入不同浓度的DOX-PBCA-NP进行培养,流式细胞仪(FCM)测定细胞凋亡和细胞周期,倒置显微镜观察细胞生长情况。结果DOX-PBCA-NP呈圆形,载药量与包封率分别为10.58%与87.43%。在同一DOX浓度的DOX组、DOX-PBCA-NP组与对照组相比,G1/G0期SHG44细胞增多、S期细胞减少(P<0.01),DOX-PBCA-NP组的变化较DOX组更明显(P<0.05)。结论用乳化聚合法制备的DOX-PBCA-NP形态一致,具有较高的载药量和包封率,体外对脑胶质瘤SHG44细胞的抑制作用较DOX增强。

Objective To prepare the doxorubicin-polybutylcyanoacrylate nanoparticles and study on its inhibition effect with SHG44 cells. Methods The DOX-PBCA-NPs were prepared using the emulsion polymerization method, nanoparticle morphology observed by transmission electron microsope. The drud loading and entrapment efficiency of doxorubicin in the nanoparticles were measured by means of UV spectra. The SHG44 cells were cultured in different concentration of DOX-PBCA-NP, cell cycles and cell apoptosis determined by flow cytometry, and the cell modalities were observed using inverse microscope. Results The DOX-PBCA-NPs were uniform spheres with drug loading and entrapment efficiency for 10.58% and 87.43% respectively. Compared with control groups, G1/G0 phase of SHG44 cells increased and S phase cells decreased in the DOX groups and DOX-PBCA-NP groups with the same DOX concentration（P 〈0. 01 ）. The changes in the DOX-PBCA-NP groups were more remarkable than DOX groups （P 〈 0. 05）. Conclusion DOX-PBCA-NPs obtained by the emulsion polymerization method were uniform morphology, beating higher drud loading and entrapment efficiency, were more effective on SHG44 cells than doxorubicin as to cell inhabition.