吡咯烷二硫氨基甲酸酯对犬体外循环中红细胞的保护作用

Protective Effect of Pyrrolidine Dithiocarbamate on Erythrocytes during Canine Cardiopulmonary Bypass

目的研究犬体外循环(CPB)中吡咯烷二硫氨基甲酸酯(PDTC)对红细胞的保护作用。方法将12只犬随机分为对照组(n=6)和PDTC组(n=6),建立CPB模型。PDTC组于CPB前静脉注射PDTC30mg/kg,对照组于CPB前静脉注射等量生理盐水。分别于CPB前、阻断30min、阻断60min、开放30min和开放60min时测定血浆白细胞介素(IL)-lβ、IL-8、游离血红蛋白(F-HB)、丙二醛(MDA)、红细胞三磷酸腺苷(E-ATP)和红细胞超氧化物歧化酶(E-SOD)水平。结果阻断后各时间点对照组的血浆IL-lβ和IL-8水平均显著高于CPB前(P<0·01),PDTC组开放后血浆IL-lβ和IL-8水平显著高于CPB前(P<0·05,P<0·01);两组血浆MDA和F-HB水平均显著高于CPB前(P<0·01);两组E-ATP和E-SOD水平均显著低于CPB前(P<0·01)。PDTC组CPB后E-ATP水平和E-SOD活性显著高于对照组(P<0·01),而血浆IL-lβ、IL-8、MDA含量和F-HB浓度均显著低于对照组(P<0·01)。结论PDTC通过减轻CPB期间脂质过氧化反应和炎性反应对红细胞起到一定的保护作用。

Objective To investigate the protective effect of pyrrolidine dithiocarbamate （PDTC） on erythrocytes during canine cardiopulmonary bypass （CPB）. Methods Twelve adult healthy dogs undergoing CPB were randomly divided into the control group （ n = 6） and the PDTC group （ n = 6）. In the PDTC group, PDTC 30 mg/kg was administered intravenously before CPB. Dogs in the control group was intravenously administering with normal saline. The levels of interleukin （IL） -1β, IL-8, malondiadehyde （ MDA）, free hemoglobin （F-HB） in plasma, erythrocyte adenosine triphosphate （E-ATP）, and erythrocyte superoxide dismutase （E-S0D） were determined before CPB, 30 and 60 minutes after aortic cross-clamping （AC）, and 30 and 60 minutes after declamping （DC）. Results In the control group, plasma levels of IL-1β and IL-8 signif- icantly increased after CPB （P 〈 0. 01 ）. In the PDTC group, plasma levels of IL-Iβ and IL-8 significantly increased after CPB （ P 〈 0. 05, P 〈 0. 01 ）. Plasma levels of MDA and F-lIB significantly increased （ P 〈 0. 01 ） and the E-ATP level and E-S0D activity significantly decreased after CPB （ P 〈 0. 01 ） in both two groups. The E-ATP level and E-S0D activity in the PDTC group at 30 and 60 minutes after AC and 30 and 60 minutes after DC were significantly higher than those in control group （P 〈0. 01 ）. liowever, the levels of IL- 1β, IL-8, MDA, and F-HB at 30 and 60 minutes after AC and 30 and 60 minutes after DC were significantly lower in the PDTC group than those in control group （ P 〈 0. 01 ）. Conclusion PDTC can protect erythrocytes by alleviating lipid peroxidation and inflammatory response during CPB.