黑色素瘤抗原-1、3基因在肝内胆管细胞癌中的表达及临床意义

Expression of Melanoma Antigen-1,3 Genes in Human Intrahepatic Cholangiocarcinoma and Its Clinical Significance

目的研究黑色素瘤抗原(MAGE)-1和MAGE-3基因在肝内胆管细胞癌(IHCC)组织中的表达,探讨MAGE-1、MAGE-3基因与IHCC患者临床指标的关系。方法RT-PCR方法检测20例IHCC患者癌组织及相应癌旁组织MAGE-1、MAGE-3基因表达,对全部RT-PCR扩增产物中目的基因片段进行DNA测序以证实其为MAGE-1、MAGE-3基因。分析MAGE-1、MAGE-3基因表达与患者性别、年龄、肿瘤直径、有无包膜、肿瘤形态以及乙肝病毒表面抗原等临床指标的关系。结果20例IHCC患者癌组织中MAGE-1、MAGE-3基因表达的阳性率分别为35%和45%,显著高于癌旁组织(0)(P<0·01)。IHCC患者癌组织中MAGE-1、MAGE-3基因表达的阳性率与患者性别、年龄、肿瘤直径、有无包膜以及乙型肝炎病毒感染等指标均无关(P>0·05);MAGE-3基因表达的阳性率与肿瘤形态有关(P<0·05)。结论MAGE-1、MAGE-3基因在IHCC患者肝癌组织中特异性高表达,MAGE-1、MAGE-3基因可能成为IHCC患者免疫治疗的攻击靶点。

Objective To explore the possibility of using melanoma antigen （MAGE） -1 and MAGE-3 gene encoding proteins as an index of potential target for immunotherapy in intrahepatic cholangiocarcinoma （IHCC） patients. Methods The expressions of MAGE-1 and MAGE-3 genes in tumor tissues and tumor adja- cent non-IHCC liver tissues were examined by RT-PCR method. The relationship between positive expression rates of MAGE-1 and MAGE-3 genes and clinical data including sex, age, tumor diameters, tumor envelope,tumor nodules number, and hepatitis B virus surface antigen were determined. Results The positive expression rates of MAGE-1 （35%） and MAGE-3 genes （45%） were significantly higher in the tumor tissues than in tumor adjacent tissues （0） （P 〈0. O1 ）. The positive expression rates of MAGE-1 and MAGE-3 genes had no relationship with the clinical data （ P 〉0. 05 ）, except the morphology of tumor （ P 〈 0. 05 ）. Conclusion The high expression rates of MAGE-1 and MAGE-3 genes in IHCC suggests the MAGE-1 and MAGE-3gene may be a target for immunotherapy in IHCC patients.