兔下颌骨牵张成骨中VEGF与NOS的相关性研究

Expression of Vascular Endothelial Growth Factor and Nitric Oxide Synthase in Mandibular Distraction Osteogenesis of Rabbit and Their Correlation

目的:研究VEGF、iNOS和eNOS在兔下颌骨牵张成骨中的表达及其相关关系;探讨NOS和VEGF的相互作用及在促进新骨生成中的作用机制。方法:日本大耳白兔20只,随机分为五组,每组4只。分别处死空白对照组、牵张后1天组、1周组、2周组、4周组动物,取牵张处骨组织做成切片,行X线和组织学观察。结果:X线下示4周后牵张处骨缺损消失,组织学示牵张处骨小梁逐渐成熟;VEGF与NOS在形成新骨中均有高水平表达,VEGF同iNOS成正相关,r=0.844,P<0.01;VEGF同eNOS成正相关,r=0.647,P<0.;01;结论:VEGF、NOS可能共同参与促进牵张成骨新骨的形成,二者可能在上诉过程相互作用,促使成骨。

Objective：To study the expression and correlation of vascular endothelial growth factor（VEGF）, inducible nitric oxide synthase（iNOS） and endothelial nitric oxide synthase（eNOS） in Mandibular Distraction Osteogenesis of Rabbit （DO）.Methods：Twenty rabbits were randomly divided into five groups, each group comprising 4 rabbites.Osteotomy on the random side of the alveolar bone was carry out.At every of I day, 1 week,2 weeks,4 weeks of DO and antitheses groups,four rabbits were sacri- ficed. Results：The radiolucency in the distraction site disappeared in 4 weeks. Histologically, the lengthened bone consisted of mature lamellar and canellous bone.Intense expression of VEGFand NOS was observed in the regenerated callus after mandibular lengthening. Expression of VEGF have a significant positive relation to iNOS,r=0.844,P〈0.01;That have a positive relation to iNOS,r=0.647,P〈 0.01. Conclusion： Both the VEGF and NOS may participate in promoting osteogenesis in the DO . They may exert interactions in promoting osteogenesis.