应用微流体混合器芯片于食品中甲醛含量快速检测

Rapid analysis of formaldehyde in food utilizing microfluidic mixer chip

本研究利用微流体芯片配合雷射激发荧光侦测法,侦测食品中不当添加物-甲醛,实验中以4-amino-3-penten-2-one(Fluoral-P)和甲醛作用,并于微流体混合器中进行反应及荧光衍生化以快速侦测。在微流体芯片中过高浓度甲醛荧光衍生物,因荧光分子基团讯号集中,易干扰全波长与荧光的侦测,因此在微流体芯片的检测以100 ppm以下为主。而在微流体混合器方面,本研究利用1:7型式不对称管道之微流体混合器,甲醛于此芯片中行荧光侦测法测定,随着甲醛浓度的降低,荧光讯号愈平缓,易出现噪声,侦测极限可达0.4 ppm且其线性度为R2=0.9954,而浓度低于1 ppm时,其S/N比表现度随之降低,此系统可提供较短分析时间、低试剂与成本消耗。

This work uses microfluidic chip with laser - induced fluorescence detection technique to detect inappropriate additives to food. In the experimental study, 4 - amino - 3 - penten - 2 - one （ Fluoral - P） and formaldehyde chemically interacted in a 3D circular microfluidic mixer while the absorption and fluorescence intensity （of formaldehyde） were confirmed via rapid detection of fluorescence derivatization. If the concentration of the formaldehyde derivatization in the microfluidic chip is too high, the full wavelength of detection can be interfered causing the detection performance to deteriorate. Therefore, the concentration of the formaldehyde for detection in the microfluidic Chip must be less than 100 ppm. This study uses a microfluidic mixer having a 1：7 type unbalanced micro - channel. When performing fluores- cence intensity detection of formaldehyde derivatization, the fluorescence signal became flatter and the presence of noise was more likely as the concentration of formaldehyde was decreased. The limitation on this concentration was about 0.4 ppm （ R2 = 0. 9954） ; if less than 1 ppm, the S/N ratio reduced accordingly. In this study, microfluidic mixer chip for rapid analysis of formaldehyde has been proven feasible, with shorter time, less reagent, and lower cost.