微流控芯片不互溶相酶催化反应显微拉曼检测

Monitoring of immiscible phase enzyme-catalyzed process in microfluid chip by Raman microscopy

生物酶的研究对生命现象本质的揭示具有极其重要的意义。通常生物酶反应的研究是在水溶液体系中进行,与其在生物体内所处的水/有机两相界面微环境不相同。通过微流控芯片可获取稳定的水/有机相界面,并利用显微拉曼光谱仪进行微区扫描实现微流控芯片两相酶反应的检测。定量考察了氰根离子在微流控芯片中水相与苯胺相之间扩散行为,并检测了辣根过氧化物酶催化联苯胺与对甲基苯胺的聚合反应。结果表明,在芯片上,非极性分子联苯胺的酶催化反应产物结构组成较极性分子对甲基苯胺更为复杂,而在普通烧杯中的反应则观察不到这个现象。

Enzyme catalyzed process is critical for revealing the virtual life. But normally enzymes were investigated in aqueous solution which quite different from the interface between water and organic molecules in vivo micro -environment. Here enzyme catalyzed reaction was carried out at the stable water/organic interface provided by the microfluidic chip, and Raman microscopy was used to monitor the reaction. The diffusion process of cyanide was investigated quantatively, and the polymerization process of benzidine and p-Toluidin were monitored. It was showed that different products from benzidine could be observed at various position in microfluidic chip, which did not happen in beaker or p - Toluidin system..