摘要
目的从没药提取物中分离纯化倍半萜单体成分,并探讨其抑制肿瘤细胞增殖的生物活性。方法没药石油醚提取部分经硅胶柱层析分离得到二十多个倍半萜类化合物。其中化合物1和2的结晶分别经熔点测定、薄层层析1、H-NMR和13C-NMR数据分析以及与已知化合物比较,确定其化学结构。利用MTT法检测化合物1和2对大肠癌细胞HT-29和激素非依赖型前列腺癌细胞PC-3、PC-3M、DU-145及正常肝的永生化细胞LO2细胞增殖的影响。结果经理化性质鉴定、波谱分析,确定化合物1的结构为[1(10)E,2R,4R]-2-甲氧基-8,12-环氧吉玛-1(10),7,11-三烯-6-酮;化合物2的结构为2-甲氧基-5-乙酰基-4-呋喃吉玛-1(10)-烯-6-酮,二者同属没药吉玛烷型倍半萜类化合物。MTT结果表明,化合物1和2对大肠癌细胞增殖的抑制作用较弱。而对三种激素非依赖型前列腺癌细胞的增殖有非常显著的抑制作用(P<0.01),且呈剂量依赖性。另外,化合物对正常肝永生化细胞的增殖无明显影响。结论经分离纯化、理化性质鉴定和波谱分析,确定了没药吉玛型倍半萜化合物1和2的结构,该化合物对前列腺癌细胞的增殖有明显的抑制作用。
Objective To determine the structures of two sosquiterpene compounds isolated from Commiphora opobalsamum, and to investigate its their anti-proliferation effect on tumor cell growth. Methods Two sesquiterpenoids were isolated from the resinous exudates of Commiphora opobalsamum. Their structures were established on the basis of physical and chemical properties, and ^1H-NMR and ^13C-NMR spectroscopy. The cytotoxicity of compounds against the colorectal cancer cell line, hormone-independent human prostate cell lines and immortalizied liver normal cell line was evaluated by MTr assay. Results Structures of two sesquiterpenoids were determined as ( 1(10) E,2R,4R)-2-methoxy-8, 12-epoxygermacra-1 (10), 7, 11-trien-6-one and 2-methoxy-5-acetoxy-furanogermacr-1 ( 10)-en-6-one. The results of MTT assay showed that both compounds had an inhibitory effect on the proliferation of hormone-independent prostate cancer cell lines in a concentration-dependent manner. No significant anti-proliferation activity was observed in colorectal cells exposed to these chemicals at low concentrations. Furthermore, less cytotoxicity of these compounds was shown by using the immortalizied liver normal cell line at concentrations tested. Condusions The structure of two germacrane sesquiterpenoids were identified, and they can significantly inhibit the proliferation of hormone-independent prostate cancer cell growth.
出处
《山东大学学报(医学版)》
CAS
北大核心
2008年第4期344-348,共5页
Journal of Shandong University:Health Sciences
基金
山东省卫生系统中青年重点科技人才建设项目
国家大学生创新训练计划
关键词
没药
吉玛烷型倍半萜
前列腺癌
细胞株
Myrrh
Germacrane Sesquiterpenoids
Prostate neplasms
Cell hnes