益肾活血胶囊对同型半胱氨酸致兔动脉粥样硬化MCP-1mRNA表达及NF-κB活化的影响

Effect of the Yishenhuoxue capsule on MCP-1 mRNA expression and NF-κB activation in atherosclerosis induced by hyperhomocysteinemia in rabbits

目的探讨益肾活血胶囊对同型半胱氨酸(Hcy)致兔动脉粥样硬化(AS)单核细胞趋化蛋白-1(MCP-1)基因表达及核因子-κB(NF-κB)活化的影响。方法将40只新西兰兔随机分为正常组、模型组、益肾活血胶囊小剂量组、益肾活血胶囊大剂量组及西药(叶酸、维生素B6、维生素B12)组,除正常组外,其他组建立高Hcy血症及AS动物模型,然后分别给予相应的药物治疗。采用高效液相色谱技术检测血浆Hcy浓度、HE染色观察血管形态学变化、实时定量PCR检测动脉壁MCP-1基因表达、Western blot分析核因子-κB抑制因子α(IκBα)蛋白含量。结果益肾活血胶囊和叶酸等维生素均可明显降低血浆Hcy水平(P<0.01),抑制血管内膜增厚,下调腹主动脉MCP-1基因表达(P<0.01),增加IκBα蛋白含量(P<0.01)。益肾活血胶囊大剂量组疗效明显优于小剂量组和西药组(P<0.05)。结论益肾活血胶囊可明显降低血浆Hcy水平,抑制Hcy诱导的动脉粥样硬化的形成,其机制可能与下调MCP-1基因表达,抑制NF-κB活化有关。

Objective To explore the effects of the Yishenhuoxue capsule （YC） on aortic monocyte chemoattractant protein-1 （MCP-1） mRNA expression and nuclear faetor-kappaB （NF-κB） activation in atherogenesis of hyperhomoeysteinemic rabbits. Methods Forty male New Zealand white rabbits were divided into five groups： the control, the model, the low dose YC, the high dose YC and the western medicine （folio acid, VitB6 and VitB12 ） group. Apart from the control group, rabbits in other groups underwent balloon catheter injuries and a high methionine diet to induce hyperhomocysteinemia and atheroscleresis by treatment with different drugs. Plasma Hcy concentration, aortic morphological changes, MCP-1 mRNA expression and expression of inhibitor of nuclear factor-kappaB （IκBα） protein in the abdominal aorta were respectively detennined by high performance liquid chromatography, HE staining, real-time quantitative PCR and western blot. Results Both YC and western medicine could decrease the plasma Hcy concentration（ P 〈 0.01 ）, attenuate the intimal thickening, down-regulate the MCP-1 mRNA expression （ P 〈0.01） and increase the Iκ Bα protein expression（ P 〈0.01）. The efficacy of YC at a high dose was superior to that of YC atalowdeseandwesternmedicinc（P〈0.05）. Conclusions YC decreases the plasma Hcy level and subsequently rverses aortic lesions induced by Hcy. The mechanisms may be related to its suppressive effects on MCP-1 expression and NF-κB activation.