摘要
目的通过检测真核细胞起始因子4E(eIF4E)在喉鳞癌中的表达,探讨其与喉鳞癌的关系。方法采用免疫组织化学法检测52例喉鳞癌组织、20例喉黏膜不典型增生组织、30例喉良性增生组织及10例正常喉黏膜组织中eIF4E基因蛋白的表达情况;用Western-blot法检测20例新鲜喉鳞癌标本的癌中心区(TC)、过渡区(TR)及病理证实的无癌区(TF)中eIF4E的表达情况。结果eIF4E在喉鳞癌中表达的阳性率为100%,显著高于对照组。eIF4E在喉鳞癌的无癌区、过渡区及中心区的表达水平呈进行性增高。结论过度表达的eIF4E是喉鳞癌恶性改变及判断其恶性程度的一个分子标志。
Objective The expression of eIF4E was studied by detecting their expression in laryngeal Squarmous cell carcinoma （LSCA2）. Methods An immunohistochemical analysis with antibody to eIF4E was performed on 52 specimens of the LSCC, 20 specimens of dysplasia, 30 specimens of hyperplasia and 10 specimens of normal mucosa; Western-blot analysis was performed on tumor core, transition core and ＂tumor-free＂ core of 20 specimens of fresh LSCA2 using anti-eIF4E antiserum. Results All 52 patients （100%） of the LSCC overexpressed eIF4E. It was remarkably higher than that in the control group. The expression of eIF4E protein level from ＂tumor-free＂ core, transition core and tumor core of LSCC appeared progressive overexpression. Conclusion Overexpression of eIF4E may be a molecular marker of malignant transformation and progression in LSCC.
