滋肾解毒中药对Ⅳ型狼疮肾炎血清诱导肾小球系膜细胞Ⅳ型胶原核酸表达的影响

Research on Effects of Method of Invigorating Kidney Heat-clearing and Detoxicating (IKHD) on Collagen IVmRNA Expression of Glomerular Mesangial Cell Induced by Type-IV LN Patients Serum

目的:观察滋肾解毒中药对Ⅳ型狼疮性肾炎(LN)血清干预下肾小球系膜细胞(GMC)中Ⅳ型胶原(ColⅣ)mRNA表达的影响,从细胞分子生物学水平探讨滋肾解毒中药治疗LN的机制。方法:结合血清药理学方法,分空白对照组、对照组血清+15%LN血清组、低、中、高浓度含药血清+15%LN血清组5组观察,RT-PCR检测GMC中ColⅣ mRNA的表达。结果:含药血清可明显抑制GMC中ColⅣ mRNA的表达,以高浓度组最显著。结论:滋肾解毒中药的含药血清在15%Ⅳ型LN患者血清诱导下可明显抑制ColⅣ mRNA的表达,从而抑制GMC增殖和细胞外基质积聚,这可能是滋肾解毒中药治疗LN的分子机理。

Objective： To observe the effects of Chinese crude drug of Invigorating the Kidney, Heat - cleating and Detoxicating （IKHD） on collagen Ⅳ （ Col Ⅳ ） mRNA expression of glomerular mesangial cell（GMC） induced by type - Ⅳ Lupus nephritis （LN）. To explore the mechanism of the method of IKHD on the level of cell and molecular biology. Methods： With the method of blood serum pharmacology, 5 groups were observed： blank, control serum ＋ 15% serum of type - Ⅳ LN patients, low, medium and high concentration serum which contained Chinese medicine of IKHD ＋ 15% serum of type - Ⅳ LN patients. The method of RT -PCR was used to detect expression of Col Ⅳ mRNA. Results： Different concentration serum containing traditional Chinese medicine of IKHD could inhibit the expression of Col Ⅳ mRNA. The effect of high concentration serum was the most notable. Conclusion： Being intervened by the 15% serum of type- Ⅳ LN patients, the serum containing traditional Chinese medicine of IKHD can inhibit the expression of Col Ⅳ mRNA. Through this, it may inhibit the proliferation of GMC and the accumulation of extracellular matrix （ECM）. This may be the molecular mechanism of TCM of IKHD curing LN.