防己黄芪汤防治肾间质纤维化的实验研究

Experimental Study of Fangji Huangqi Tang to Renal Interstitial Fibrosis

目的:了解防己黄芪汤对肾间质纤维化的作用,以及去除甘草后对其作用的影响。方法:采用大鼠单侧输尿管结扎(UUO)模型,用防己黄芪汤以及防己黄芪去甘草汤干预,观察肾功能水平、肾病理以及肾α-平滑肌蛋白(α-SMA)和纤维连接蛋白(FN)蛋白和基因表达。结果:防己黄芪汤可降低UUO大鼠血尿素氮,使血白蛋白升高;减轻肾小管间质纤维化程度;同时,显著降低肾小管和肾间质α-SMA、FN的蛋白和基因表达。去甘草汤组与防己黄芪汤一样,改善了肾间质纤维化的多项指标,而且从数据来看,有进一步下降血尿素氮、以及FN表达的趋势。结论:防己黄芪汤具有抑制肾纤维化、改善肾功能的作用。组方去除甘草后没有影响这一作用,甚至有可能更好。

Objective ： Fangji Huangqi Tang is Chinese traditional medicine, used in renal diseases for thousands years. It has not studied whether it influence renal interstitial fibrosis, It include Fangji, Huangqi, Baishu ,Gancao, Fangji and Huangqi ,which can improve renal interstitial fibrosis ,while Gancao is not reasonable in renal disease ,because of increaseing edema and declining renal damage which caused by Guanmutong. It needs to be proved ifFangji Huangqi Tang can prevent the renal interstitial fibrosis, and the medicine of Fangji Huangqi Tang without Gancao has the same effect. Methods ： Rats were given UUO to make the model of renal interstition fibrosis. The rats were treated by Fangji Huangqi Tang , and the medicine of Fangji Huangqi Tang without Gancao. Monopril were regrded as control. 14 days later, the kidney samples of UUO were stained in HE and Masson. The expression of protein and mRNA of α - smooth muscle actin （α- SMA） and Fibronectin （Fn） were examined by immunohistochemical stain and by RT- PCR. Results： Fangji Huangqi Tang can reduce plasma urea nitrogen and increase plasma albumin of the rats of UUO significantly, meanwhile in improve the injury of the renal interstition significantly. The expression of both α- SMA and Fn decrease significantly treated by Fangjl Huangqi Tang. Fangji Huangqi Tang without Galcao can also reduce the injury of the renal interstition and expression of protain and mRNA of α - SMA and Fn,moreover it seems that it could have better effect in renal interstition fibrosis. Conclusion ： Fangji Huangql Tang can prevent the renal interstitial fibrosis. Fangji Huangqi Tang without Gancao has the same effect, maybe it improve renal interstitiov fibrosis significantly compared with Fangji Huangqi Tang.