HERG钾通道对调节性细胞容量下降的作用

Contribution of the HERG potassium channels to the regulatory volume decrease

目的研究HERG钾通道在细胞容量调节中的作用,并初步探讨其机制。方法应用时滞显微摄影技术检测HEK293细胞和HERG-HEK293细胞在不同渗透压时的细胞容量。结果①在低渗外液中,经过调节性细胞容量下降(RVD)过程,HEK293细胞RVD率为(77.8±3)%(n=20,P<0.01);HERG-HEK293细胞RVD率为(174±2.6)%(n=20,P<0.01)。容量调节性氯通道阻断剂NFA 10nmol/L、DIDS 100μmol/L,对上述2种细胞RVD过程均有明显抑制作用;②加HERG通道阻断剂Cisapride 100nmol/L后,HERG-HEK293细胞RVD率为(47.4±5)%(n=20,P<0.01)。结论HERG通道可能部分参与细胞容量调节,可能是调节性细胞容量下降的机制。

Objective To investigate the contribution of human ether-a-gogo related gene（HERG） potassium channels to the regulatory volume decrease（RVD）and the related mechanism.Methods Lag-time microphotography was employed to detect the cell volumes of HEK293 cells and HERG-HEK293 cells.Results Exposing to the hypotonic solution,the RVD rate of HEK293 cells and HERG-HEK293 cells were（77.8±3）%（n=20,P〈0.01） and（174±2.6）%（n=20,P〈0.01）,respectively.In contrast,treatment with 10nmol/L Niflumic acid and 100μmol/L DIDS resulted in the inhibition of the RVD process.HERG channel blocker Cisapride 100nmol/L also depressed the RVD process,and the RVD rate was（47.4±5）%（n=20,P〈0.01）.Conclusion HERG potassium channels are partly involved in the regulatory volume decrease,and it might be a mechanism for the regulatory volume decrease of HERG potassium channels.