摘要
目的观察蛋白激酶C(PKC)抑制剂chereythrine对大鼠皮层神经元延迟整流钾电流(IK)的影响。方法采用全细胞式膜片钳技术,检测了chereythrine对大鼠皮层神经元IK的影响。结果①chereythrine(20μmol/L)对大鼠皮层神经元IK有抑制作用,抑制率为(41.2±9.62)%(P<0.01);②chereythrine(20μmol/L)可使大鼠皮层神经元IK的电流-电压曲线下移,并呈现一定的电压依赖性。结论PKC抑制剂chereythrine对大鼠皮层神经元IK具有抑制作用。
Objective To study the inhibition of chereythrine,a protein kinase C(PKC) inhibitor,on the delayed rectifier-like potassium current(IK) in cerebral cortical neurons of rats.Methods Whole-cell voltage clamp technique was used.Results Chereythrine(20μmol/L) inhibited the IK of freshly dissociated cerebral cortical neurons(P〈0.01).At the concentration of 20 μmol/L,the amplitudes of IK decreased at different membrane potentials by chereythrine,IK decreased in a voltage-dependent manner.Conclusion It suggests that chereythrine can inhibit the IK in cerebral cortical neurons of rats.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2008年第2期111-113,共3页
Journal of Harbin Medical University
基金
黑龙江省教育厅基金资助项目(11521120)
黑龙江省卫生厅课题(2005-27)
关键词
蛋白激酶C
皮层
全细胞式膜片钳技术
延迟整流钾电流
protein kinase C
cortex
whole-cell voltage clamp technique
delayed rectifier-like potassium currents