PKC抑制剂chereythrine对大鼠皮层神经元延迟整流钾电流的抑制作用

Inhibition of chereythrine,a PKC inhibitor,on the delayed rectifier-like potassium current in cerebral cortical neurons of rats

目的观察蛋白激酶C(PKC)抑制剂chereythrine对大鼠皮层神经元延迟整流钾电流(IK)的影响。方法采用全细胞式膜片钳技术,检测了chereythrine对大鼠皮层神经元IK的影响。结果①chereythrine(20μmol/L)对大鼠皮层神经元IK有抑制作用,抑制率为(41.2±9.62)%(P<0.01);②chereythrine(20μmol/L)可使大鼠皮层神经元IK的电流-电压曲线下移,并呈现一定的电压依赖性。结论PKC抑制剂chereythrine对大鼠皮层神经元IK具有抑制作用。

Objective To study the inhibition of chereythrine,a protein kinase C（PKC） inhibitor,on the delayed rectifier-like potassium current（IK） in cerebral cortical neurons of rats.Methods Whole-cell voltage clamp technique was used.Results Chereythrine（20μmol/L） inhibited the IK of freshly dissociated cerebral cortical neurons（P〈0.01）.At the concentration of 20 μmol/L,the amplitudes of IK decreased at different membrane potentials by chereythrine,IK decreased in a voltage-dependent manner.Conclusion It suggests that chereythrine can inhibit the IK in cerebral cortical neurons of rats.