CD44v6在喉和下咽鳞状细胞癌组织中的表达及其临床意义

Expression and clinical significance of CD44v6 in tissues of laryngeal and hypopharyngeal squamous cell carcinoma

目的设计制作喉和下咽鳞状细胞癌组织芯片,应用组织芯片免疫组化技术探讨细胞黏附分子CD44v6的表达与喉和下咽鳞状细胞癌侵袭转移等生物学行为的相关性。方法选取手术切除的喉和下咽鳞状细胞癌原发灶、癌旁正常黏膜和淋巴结转移癌组织共125例,甲醛固定、石蜡包埋,利用这些石蜡标本制作组织芯片;应用免疫组化技术在组织芯片上检测黏附分子CD44v6的表达,分析其表达率与喉癌和下咽癌临床和病理分期的相关性。结果CD44v6在原发癌中的阳性表达率明显高于癌旁正常组织(P<0.05),淋巴结转移癌中表达率明显高于癌旁正常组织(P<0.001)和原发癌(P<0.01);有淋巴结转移组CD44v6表达率明显高于无淋巴结转移组(P<0.01),高分化组明显低于中分化组(P<0.05),而CD44v6的表达率与肿瘤的原发部位、浸润范围、年龄、性别等因素无明显相关。结论CD44v6在喉癌和下咽癌组织中高表达,其表达率与肿瘤的分化程度和淋巴结转移有关,可作为临床预测喉和下咽鳞状细胞癌颈淋巴结转移趋势和估计预后的主要参考指标之一。

Objective To design and prepare a tissue microarray of laryngeal and hypopharyngeal squamous cell carcinoma（LHSCC） and study CD44v6 expression in larynx and hypopharynx carcinoma by immunohistochemistry and analyze the relationship between CD44v6 expression and biological behavior of LHSCC using the tissue microarray.Methods One hundred and twenty five tissues of primary cancer,normal tissue,and metastatic carcinoma were obtained during operation.These tissues were fixed in formalin and were embedmented with paraffin.A tissue microarray was made by using these paraffin-embedded tissues.The expression of CD44v6 in laryngeal and hypopharyngeal squamous cell carcinoma was studied by immunohistochemistry in the tissue microarray,and the clinical significance of CD44v6 expression was analyzed.Results In primary cancer tissue,the expression rate of CD44v6 was significantly higher than that in normal tissue（P〈0.05）;the rate in lymph node metastatic carcinoma was significantly higher than that in normal tissue（P〈0.001）and primary cancer tissue（P〈0.01）;CD44v6 expression rate of the group with lymph node metastasis was significantly higher than that in group without lymph node metastasis（P〈0.01）;the expression rate of the well-differentiated group was higher than that in moderately differentiated group（P〈0.05）.Conclusion There is an overexpression of CD44v6 in larynx and hypopharynx carcinoma,the expression rate was concerned with degree of cell differentiation and metastasis of lymph node.CD44v6 overexpression may become a predict index of metastasis and prognosis of laryngeal and hypopharyngeal squamous cell carcinoma.