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腺病毒介导的人CD40配体基因抑制裸鼠卵巢癌生长的实验研究

EXPERIMENTAL STUDY OF ADENOVIRUSMEDIATED HUMAN CD40 LIGAND GENE IN INHIBITING THE GROWTH OF NUDE MICE'S OVARIAN CANCER
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摘要 [目的]探讨重组腺病毒人CD40配体基因(Ad-hCD40L)对卵巢癌的治疗作用。[方法]建立卵巢癌裸鼠动物模型,检测腺病毒介导的人CD40配体基因对肿瘤生长的抑制作用。[结果]瘤内注射Ad-hCD40L明显抑制裸鼠卵巢癌生长,Tunel法检测和HE染色肿瘤组织石腊切片观察调亡细胞明显增多,并可见大量肿瘤细胞坏死。[结论]腺病毒介导的人CD40配体基因抑制裸鼠肿瘤生长,增强诱导肿瘤细胞调亡,为卵巢癌的基因治疗提供了新的途径。 [Objective] To investigate the inhibitory, effect in treating ovarian cancer with recombinant adenovirusmediated human CD40 ligand gene （Ad-hCD40L）. [Methods] A nude mice＇s ovarian cancer model was built to exam the andcancer effect of recombinant Ad-hCD40 on the tumor growth. [ Results] Human CD40 ligand gene could suppress the growth of tumor in nude mice by intratumoral injection with Ad-hCD40L. Immunohistochemical and HE staining revealed that in Ad-hCD40L treatment group apoptosis obviously increased and a great of tumor cells occurred necrosis comparing with other groups. [Conclusion] The recombinant Ad-hCD40L could suppress the growth of tumor and induce more apoptosis and necrosis in ovarian cancer ceils. These findings may provide a new strategy in treating ovarian cancer with gene therapy.
作者 吴红兵 田聆 王虎 文艳君 魏于全 郑广宁
机构地区 四川大学华西口腔医院放射科 四川大学华西医院生物治疗国家重点实验室
出处 《现代预防医学》 CAS 北大核心 2008年第9期1736-1737,1740,共3页 Modern Preventive Medicine
基金 国家自然基金资助项目(10675089)
关键词 人CD40配体 重组腺病毒载钵 裸鼠卵巢癌 基因治疗 Human CD40L Recombinant adenovirus vector Nude mice＇s ovanan cancer Gene therapy
分类号 R737.31 [医药卫生—肿瘤]
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参考文献13

  • 1Tanja Hakkarainen, Akseli Hemminki, Alexander V. Pereboev et al. CD40 Is Expressed on Ovarian Cancer Cells and Can Be Utilized for Targeting Adenoviruses [J]. Clinical Cancer Research, 2003, 9 : 619-624.
  • 2Jamal A, Tiwari RC, Murray T, et al. Cancer statistics, 2004 CA [J]. Cancer J Clin, 2004, 54 (1): 8-29.
  • 3Rosenberg SA. Progress in human tumour immunology and immunotherapy[J]. Nature, 2001, 411: 380-38.
  • 4Smyth MJ, Godfrey DI, Trapani JA. A fresh look at tumor immunosurveillance and immunotherapy [J]. Nat Immunol, 2001, 2 (4) : 293-299.
  • 5吴红兵,田聆,文艳君,刘玉梅,阚兵,杜小波,徐健蓉,魏于全.表达人CD40配体的重组腺病毒载体的构建[J].华西口腔医学杂志,2005,23(6):534-536. 被引量：1
  • 6Pandori M, Hobson D, Sano T. Adenovirus-microbead conjugates possess enhanced infectivity: a new strategy for localized gene delivery[J]. Virology, 2002, 299: 204-212.
  • 7Clark LB, Foy TM, Noene RJ, et al. CD40 and its ligand[J]. Adv Immunol, 1996, 63: 43-78.
  • 8Vestal RE, WingettDG, Knight LK, et al. Expression of CD40 in breast, colon, lung and ovarian tumors[J]. Proc AM A ssocCancer Res, 1997, 38: 1456-1550.
  • 9Ghammnde S, Hylander BL, Otlazoglu E, et al. Recombinant CD40 ligand therapy has significant antitumor effects on CD40- positive ovarian tumor xenografts grown in SCID mice and demonstrates an augmented effect with cisplatin [J]. Cancer Res, 2001, 61: 7556-7562.
  • 10Pulten SS, Miller HG, Everdeen DS, et al. CD40- tumor necrosis factor receptor-associated factor (TRAF) interactions: regulation of CD40 signaling through multiple TRAF binding sites and TRAF hetero-oligomefization [J]. Biochemistry, 1998, 37: 11836- 11845.

二级参考文献11

  • 1Schonbeck U,Libby P.The CD40/CD154 receptor/ligand dyad[J].Cell Mol Life Sci,2001,58(1):4-43.
  • 2Pandori M,Hobson D,Sano T.Adenovirus-microbead conjugates possess enhanced infectivity:A new strategy for localized gene delivery[J].Virology,2002,299(2):204-212.
  • 3Nasz I,Adam E.Recombinant adenovirus vectors for gene therapy and clinical trials[ J ].Acta Microbiol Immunol Hung,2001,48 (3-4):323-348.
  • 4Adam E,Nasz I.Adenovirus vectors and their clinical application in gene therapy[J].Orv Hetil,2001,142(38):2061-2070.
  • 5Tong-chuan HE,Shibin Z,Luis T.A simplified system for generating recombinant adenoviruses[J].Proc Natl Acad Sci USA,1998,95(5):2059-2514.
  • 6Briones J,Timmerman J,Levy R.In vivo antitumor effect of CD4OLtransduced tumor cells as a vaccine for B-cell lymphoma[J].Cancer Res,2002,62 (11):3195-3199.
  • 7Loskog A,Totterman TH,Bohle A.In vitro activation of cancer patient-derived dendritic cells by tumor cells genetically modified to express CD154[J].Cancer Gere Ther,2002,9(10):846-853.
  • 8Kikuchi T,Crystal RG.Anti-tumor immnunity induced byin vivo adenovirus vector-mediated expression of CD40 ligand in tumor cells[J].Hum Gene Ther,1999,10(8):1375-1387.
  • 9Sun Y,Pend D,LecandaJ.In vivo gene transfer of CD40 ligand into corlon cancer cells induces local production of cytokines and chemokines.Tumor eradication and protective antitumor immunity[J].Gene Ther,2000,7 (17):1467-1476.
  • 10Schmitz V,Barajas M,Wang L,et al.Adenovirus-mediated CD40 ligand gene therapy in a rat model of orthotopic hepatocellular carcinoma[J].Hepatology,2001,34(1):207-209.
现代预防医学
2008年 第9期

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