闭合性颅脑损伤早期大鼠海马差异蛋白质研究

Analysis of differential expression of proteins in rat hippocampus following early closed brain injury

【目的】研究大鼠闭合性颅脑损伤后24h海马中蛋白质表达的变化及其特点。【方法】选成年雄性sD大鼠随机分为手术对照组及损伤后24h组，复制Marmarou落体打击闭合性颅脑损伤大鼠模型，应用双向电泳技术分析大鼠脑损伤后24h海马中蛋白质表达的改变。【结果】双向电泳分离结果显示，采用pH3～10胶条，与手术对照组比较，损伤后24h有7个蛋白质点有表达量上的差异，并有2个蛋白质点消失，新出现1个蛋白质点；采用DH4～7胶条，与手术对照组比较，损伤后24h有7个蛋白质点有表达量上的差异，并有1个蛋白质点消失，新出现3个蛋白质点。【结论】大鼠闭合性颅脑损伤早期（24h）可引起海马蛋白质表达发生变化。

[Objective] To investigate the alteration of hippocampus protein expression of rats after closed brain injury. [Methods] Male Sprague-Dawley rats were randomly divided into sham operation group and 24 h after injury group. The head injury animal model was produced by an weight-drop device designed by Marmarou, and the change of protein expression in hippocampus was detected by two-dimensional electrophoresis. [Results] In pH3 - 10 stnp, in 24 h post- injury group, there were 7 protein spots showed significant difference as compared with sham operation group. Meanwhile, 2 protein spots disappeard and 1 protein spots emerged. In pH 4 ~ 7 strip, a total of 7 protein spots 24 h post - injury group showed significant difference, moreover, 1 protein spot disappeared, and 3 protein spots emerged. [Conclusions] The alteration of protein expression in hippocampus could be induced by closed brain injury.