神经生长因子对哮喘豚鼠支气管肺泡灌洗液中IL-1β及IL-4的影响

Effects of NGF on IL-1β and IL-4 in BALF in Guinea Pig Models of Asthma

目的探讨神经生长因子(nerve growth factor,NGF)对哮喘豚鼠支气管肺泡灌洗液(BALF)中IL-1β、IL-4的影响。方法将30只豚鼠随机分为3组,哮喘组豚鼠采用卵清蛋白(OVA)致敏和雾化吸入激发的方法制成哮喘模型;anti-NGF抗体组豚鼠致敏和激发方法同哮喘组,但在激发前3h腹腔注射anti-NGF抗体;正常对照组豚鼠用PBS腹腔注射和雾化吸入。最后一次OVA激发后24h内,行支气管肺泡灌洗,观察BALF中炎性细胞的变化,酶联免疫吸附试验(ELISA)检测BALF上清中IL-1β、IL-4的水平。结果anti-NGF抗体组BALF中炎性细胞总数、嗜酸粒细胞、巨噬细胞和淋巴细胞计数与正常对照组及哮喘组比较,差异均有统计学意义(P<0.05);哮喘组BALF上清中IL-1β、IL-4水平与哮喘组及正常对照组比较,差异亦均有统计学意义(P<0.05)。结论NGF对IL-1β、IL-4有调节作用,参与了哮喘发病的免疫炎症过程。

Objective To explore the effects of nerve growth factor （NGF） on IL - 1 β AND IL - 4 in bronchoalveolar lavage fluid （BALF） in guinea pig models of asthma. Methods Thirty guinea pigs were randomly allocated to three groups： normal group; asthma group ; anti - NGF group. The asthmatic models were established by injection and inhalation of OVA, the anti - NGF models by injection of anti - NGF antibody peritoneally three hours before inhalation OVA, and the animals in the control group were given PBS by both injection and inhalation. At 24 hour after the last OVA challenge, BALF was collected. Inflammatory cells in BALF were counted ; the levels of IL - 1 β and IL - 4 were measured by enzyme - linked immunosorbent assay （ELISA）. Results Compared with the control group and asthma group, the total numbers of inflammatory cells, the counts of eosinophils, macrophage and lymphocyte of BALF in anti - NGF group showed significant differences （ P 〈 0. 05 ）. The levels of IL - 1β and IL - 4 of BALF in asthma group were significantly different from those in the control and anti - NGF groups （ P 〈 0.05 ）. Conclusion NGF can regulate the levels of IL - 1 β and IL - 4 of BALF in asthma models of guinea pig, NGF may contribute to immune inflammation in asthma.