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高压诱导小蛋白Trpcage变性的研究

Study of High Pressure-induced Trpcage Denaturation
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摘要 在温度为300 K,压强为2000 bar、10000 bar时,对小蛋白Trpcage的去折叠过程进行了分子动力学模拟,模拟中考虑了水的压缩系数随压强改变情况.结果发现,高压会导致Trpcage变性.然后对其变性原因进行了分析. The molecular dynamical simulation was performed at 300K and the pressure of 2000bar,10000bar to study the unfolding of miniprotein Trpcage, the total simulating time is more than 100ns and the changes of the compressibility are considered during the simulation. The results show high pressure can induce the miniprotein denaturation, then the causes are analyzed based on these.
作者 于家峰 窦相华
机构地区 山东省高校生物物理重点实验室
出处 《德州学院学报》 2008年第2期9-11,14,共4页 Journal of Dezhou University
基金 山东省自然科学基金(Y2005D12)
关键词 高压 分子动力学模拟 变性 Trpcage high pressure molecular dynamical simulation denaturation Trpcage
分类号 Q61 [生物学—生物物理学]
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参考文献18

  • 1van gunsteren WF, Hunenberger PH, Kovacs H,et al. Investigation of protein unfolding and stability by computer simulation[J]. Phil Trans R Soc Lond B, 1995, 348:49-59.
  • 2Zhou YQ, Karplus M. Folding thermodynamics of a model three-helix-bundle protein [J]. Proc Natl Acad Sci USA, 1997,94:1429-1443.
  • 3Vijay SP, Daniel SR. Molecular dynamics simulation of unfolding and refolding of a β- hairpin fragment of protein G[J]. Proc Natl Acad Sci USA, 1999,96:9062-9067.
  • 4Carlos S, Bentley S, Andrian ER. All-atom structure prediction and folding simulations of a stable protein [J]. J Am Chem Soc, 2002,124:11258-11259.
  • 5Kundrot C. E, Richards F.. M. Crystal structure of hen egg-white lysozyme at a hydrostatic pressure of 1 000 atm[J]. J. Mol. Biol., 1987,193:157-170.
  • 6Kundrot C. E, Richards F.. M. Effect of hydrostatic pressure on the solvent in crystals of hen egg-white lysozyme[J]. J. Mol. Biol. , 1988,200:401 - 410.
  • 7Berthold Wroblowski, Jose Fernando Diaz, Karel Heremans,et al. Molecular mechanisms of pressure induced conformational changes in BPTI[J]. PROTEINS:structure, Function, and Genetics, 1996,25:446-455.
  • 8Nathan Hillson, Jose Nelson Onuchic, et al. Pressure protein - folding/unfolding kinetics [J]. PNAS, 1999, 26: 14848-14853.
  • 9Hua Li, Hiroaki Yamada, Kazuyuki Akasaka. Effect of pressure on the tertiary structure and dynamics of folded basic pancreatic trypsin inhibitor[J]. Biophysical Journal, 1999,77:2801-2812.
  • 10Kenji Sasahara, Masao Sakurai, Katsutoshi Nitta. Pressure effect on denaturant-induced unfolding of Hen Egg White Lysozyme[J]. PROTEINS: structure, Function, and Genetics,2001,44:180-187.
德州学院学报
2008年 第2期

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