银杏产黄酮内生真菌的分离与鉴定

Isolation and Identification of Endophytic Fungi of Falvonoid-Producing Ginkgo biloba

为实现真菌发酵生产黄酮,用HPLC和氯化铝比色法,从四川、贵州、山东等地采集的银杏根、茎、叶中分离到7株产黄酮真菌。分子和形态学鉴定结果表明:这些菌株分别属于Colletotrichum和Fusarium,菌株QC102经鉴定为Colletotrichumacutatum Simmonds,其总黄酮产量为8.2μg/mL。

Seven fungal isolates were found to produce falvonoids which were acquired from root, stem and leaves of Ginkgo biloba L. collected in the areas of Sichuan, Guizhou and Shandong provinces in Chi- na by means of HPLC and AlCl3 chromatometry. The further molecular and morphological identification indicated that these fungal isolates belonged to Colletotrichum and Fusarium respectively. The isolate QC102 was indentified as C. acutatum Simmonds which can produce falvonoids up to 8.2μg/mL.