不同共培养细胞类型对绵羊孤雌生殖胚发育的影响

Effect of different co-culture cell types on the development of ovine parthenogenetic embryos

体外成熟的绵羊卵母细胞经孤雌激活4h后,分别在5种体细胞单层(成熟前颗粒细胞、成熟后颗粒细胞、输卵管壶腹部上皮细胞、输卵管峡部上皮细胞、子宫内膜细胞)中进行共培养,比较其对绵羊孤雌生殖胚体外发育的影响。结果表明:成熟前后颗粒细胞对绵羊孤雌胚体外发育的卵裂率和囊胚率差异不显著(P>0·05);但输卵管壶腹部上皮细胞(27·5%)、子宫内膜细胞组囊胚率(27·7%)高于输卵管峡部上皮细胞和颗粒细胞(17·7%、20·3%),且差异显著(P<0·05);壶腹部上皮细胞与子宫内膜细胞在囊胚率上差异不显著(P>0·05)。说明在绵羊孤雌生殖胚体外发育中,选择成熟前及成熟后的颗粒细胞都可达到相同的共培养效果;使用输卵管壶腹部上皮细胞、子宫内膜细胞进行共培养效果优于输卵管峡部上皮细胞和颗粒细胞,可以得到较高的囊胚率。

After matured ovine oocytes were activated by 5μmol/L A23187 for 5 rain and then 2 mmol/L 6 - DMAP for 4 h, it was cultured in vitro in five types of cell monolayers （ including pre - matured granulosa cells, matured granulosa cells, oviduct ampulla epithelium cells, oviduct isthmus epithelium cells and endometrium cells） respectively. The cleavage rates and blastocyst rates of embryos treated between pre -matured granulosa cells and matured granulosa cells, were not significantly different ; and the blastocyst rates of oviduct ampulla epithelium cell and eedometrium cell groups（27.5 % ,27.7% ）, were significantily higher than the others of oviduct isthmus epithelium cell and granulosa cell groups（ 17.7% ,20.3% ） （ P 〈 0. 05 ）. These results indicated that the pre - matured granulosa and matured granulosa cells may play the same role on the development of ovine parthenogenetic embryos. The oviduct ampulla epithelium cells and endometrium cells are suitable for the development of ovine parthenogenetic embryos cultured in vitro, and their blastocyst rates reach to 27.5% and 27.7% respectively