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低能量激光照射对颌骨新生作用的组织化学测定 被引量：1

The PCNA study of the mandibular bone regeneration by low energy laser irradiation
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摘要 目的探讨低能量激光对于颌骨新生过程中成骨细胞增殖的影响。方法选择健康成年杂种狗5只,于两侧颏孔区形成骨折,右侧以低能量激光照射骨折区,每日一次10min(每日总剂量108J),持续12d,采用免疫组化抗体PCNA测量骨折边缘新生骨细胞增生量。结果两侧均有PCNA阳性反应细胞,右侧骨折缺损区正反应细胞大量增生,与左侧对照组相比差异有显著性(P<0.001)。结论低能量激光能刺激和促进骨新生,有利于骨折愈合。 Objective To study the effect of low energy laser irradiation on cellular proliferation of the mandibular fracture. Methods To detect the initial change. Five-beagle were used in this study,the had been fractured the bilateral mandibular. Low energy laser irradiation was applied on fight side to irradiate the fractured area for 10 minutes per day for 12 days （total energy：108 J/d）. Immunohistochemical staining of proliferating cell nuclear antigen （PCNA） was performed to evaluate celluar proliferation on bone margin. Results The bilateral fractured area have cellular proliferation, the laser irradiation group presented the higher cellular proliferation rate PCNA-Positive （P〈0.001） than that of nonirradiation group. Conclusion The effect of study suggests that the low energy laser irradiation can stimulate and accelerate the bone regeneration.
作者 王元银 陈乔尔 王银龙 何家才 刘文清 杨晨 程继光 徐文华 张娟 朱坤鹍 胡超
机构地区 安徽医科大学附属口腔医院口腔外科 安徽医科大学附属口腔医院口腔组织病理科 中国科学院安徽光机所
出处 《安徽医科大学学报》 CAS 北大核心 2008年第2期160-162,共3页 Acta Universitatis Medicinalis Anhui
关键词 激光/治疗应用 骨再生 laser/therapeutic use bone regeneration
分类号 R454.2 [医药卫生—治疗学] R336 [医药卫生—人体生理学]
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参考文献10

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同被引文献8

  • 1Eissa M M,Soliman A S,Nassar S O.Ultrastructural and immunological features of experimental cutaneous leishmaniasis after treatment with intralesional hypertonic sodium chloride and CO2 laser rays[J].J Egypt Soc Parasitol,2003,33(1):329-52.
  • 2Kujawa J,Zavodnik L,Zavodnik I,et al.Low-intensity near-infrared laser radiation-induced changes of acetylcholinesterase activity of human erythrocytes[J].J Clin Laser Med Surg,2003,21(6):351-5.
  • 3Kreisler M B,Haj H A,Noroozi N.Willershausen B.Efficacy of low level laser therapy in reducing postoperative pain after endodontic surgery-a randomized double blind clinical study[J].Int J Oral Maxillofac Surg,2004,33(1):38-41.
  • 4Giliazetdinova IuA,Vinnichenko A V,Vinnichenko IuA.Magnetic laser therapy in the treatment of apical periodontitis[J].Stomatologiia,2003,82(4):20-4.
  • 5Milojevic M,Kuruc V.Low power laser biostimulation in the treatment of bronchial asthma[J].Med Pregl,2003,56(9-10):413-8.
  • 6Battle E F Jr,Hobbs L M.Laser therapy on darker ethnic skin[J].Dermatol Clin,2003,21(4):713-23.
  • 7Guzzardella G A,Torricelli P,Nicoli-Aldini N,et al.Osseointegration of endosseous ceramic implants after postoperative low-power laser stimulation:an in vivo comparative study[J].Clin Oral Implants Res,2003,14(2):226-32.
  • 8Toida M,Watanabe F,Goto K,et al,Usefulness of low-level laser for control of painful stomatitis in patients with hand-foot-and-mouth disease[J].J Clin Laser Med Surg,2003,21(6):363-7.

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安徽医科大学学报
2008年 第2期

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