多菌灵对茶树菇菌丝生长的影响及其在子实体中的残留

Effects of Carbendazim on Mycelial Growth of Agrocybe aegerita and Residue in Fruit Body

研究了多菌灵对茶树菇和食用菌生产中3大主要污染霉菌菌丝生长的影响。在PDA培养基上多菌灵对茶树菇菌丝和孢菌、木霉及曲霉菌丝的EC50分别为22.3μg·mL-1、0.06μg·mL-1、0.29μg·mL-1和0.64μg·mL-1,MIC分别为60μg·mL-1、0.9μg·mL-1、1.6μg·mL-1和2.0μg·mL-1;在出菇袋料培养基中,按1∶500、1∶1000和1∶2000添加多菌灵对茶树菇菌丝生长影响不明显。同时采用液相色谱法分析了多菌灵在茶树菇子实体中的残留,3种处理的残留量分别为0.09ppm、0.04ppm和0.01ppm。

Effects of Carbendazim on mycelial growth of Agrocybe aegerita and three main polluting mould including Neurospora sp., Trichoderma sp. and Aspergillus sp. in edible fungi produce was studied. The ECs0 and MIC were 22.3μg·mL^-1, 0.06μg·mL^-1, 0.29μg·mL^-1, 0.64μg·mL^-1 and 60.00μg·mL^-1, 0.90μg·mL^-1, 1.60μg·mL^-1, 2.00μg·mL^-1 to them on PDA medium, respectively. No obvious effects on mycelial growth of Agrocybe aegerita were observed in the three rates of Carbendazim and fruit body growing medium （ 1： 500, 1：1 000 and 1：2 000） . Meanwhile, Carbendazim residue in fruit body was analyzed by HPLC, the amounts of residue in three treatments were 185μg·g^-1, 74μg·g^-1and 12.3 μg·g^-1, respectively.