超声波-超低温冻融处理对灵芝孢子粉多糖提取的影响

Effect on the Distill of Polysaccharides in Ganoderma lucidum Spore Powder Treated with Ultrasonic and Thawing and Freezing Test of Ultra-low Temperature

利用苯酚-硫酸法对破壁处理后的灵芝孢子粉多糖含量进行测定,结果表明采用超低温冻融处理和超声波处理进行灵芝孢子粉破壁均具有效果好、成本低及操作简便等特点;增加冻结-解冻循环次数在一定程度上能提高多糖含量,循环次数以5次～7次为宜;将超声波处理法和超低温冻融法很好地结合起来,其多糖释放更高,效果更佳,此方法更具实用价值。

The measurement of polysaccharides in sporoderm-broken spore was analysed by the method of phenol-sulfuric acid. The result shows that it is an effective, economic and facile way to break the cellular wall with thawing and freezing of ultra-low temperture or ultrasonic. And the circulating times of thawing and freezing can increase the contents of polysaccharides in a certain degree and the suggested number is 5-7. The method of combining ultrasonic with thawing and freezing of ultra-low temperture commendably can release the polysaccharides of its contents higher and be of more practical value.