灰黄霉素高产变株与出发菌株18S rRNA基因序列的比较分析

Comparative Analysis of 18S rRNA Gene Sequences between the High Griseofulvin Producing Mutant and Its Original Strain

根据真菌18S rDNA的保守序列设计引物,对灰黄霉素高产突变菌Penicillium griseofulvum F208与出发菌株Penicillium griseofulvum 3.5190的18S rRNA进行克隆测序及对比分析,并登录GenBank(序列号为EF608151和EF607282).依据18S rDNA建立的系统进化树表明突变株F208与出发菌株3.5190的遗传距离较远,而与Penicillium urticae亲缘关系最近.出发菌株3.5190与Penicillium commune亲缘关系最近.18SrDNA作为分子标记可有效地鉴别灰黄霉素产生菌(Penicillium griseofulvum)高产与低产菌株.

18S rRNA genes of the high griseofulvin producing mutant Penicillium griseofulvum F208 and its original strain PeniciUium griseofulvum 3. 5190 were successfully isolated by PCR with the primers designed on the basis of the consecutive 18S rDNA sequences of fungi. Their sequences were submitted to GenBank （Accessoin No. EF608151 and EF607282）. The phylogenetic tree based on 18S rDNA sequences showed that the evolutionary distance between the mutant F208 and the original strain 3. 5190 was far away. The mutant F208 was closed to Penicillium urticae, but the original strain 3. 5190 was closed to Penicillium commune. 18S rDNA could be used as a molecular maker to identify the difference between the original and the mutant highly producing griseofulvin.