烯丙异噻唑刺激稻株体内超氧自由基产生与水稻抗稻瘟病的关系

EFFECT OF PROBENAZOLE ON THE SUPEROXIDE ANION RADICAL IN RICAAND RELATION TO RESISTANCE AGAINST FICE BICE BLAST DISEASE

超氧自由基（Ｏ＿２）清除剂甘露醇和抗坏血酸在离体条件下对稻瘟菌丝生长有一定抑制作用，但它们施用到水稻上后却会加重稻瘟病的发生。Ｏ＿２清除剂对烯丙异表现拮抗作用。经烯丙异处理的水稻幼苗接种稻瘟菌后能刺激Ｏ＿２的产生。外源Ｈ＿２Ｏ＿２和超氧自由基对稻瘟病菌和水稻细胞有直接毒害。本研究表明：烯丙异刺激水稻体内产生的Ｏ＿２在药剂诱导水稻对稻瘟病抗性反应中发挥作用。

O2 scavengers mannitol and ascobic acid showed inhbitio. to mycelial growth of Pyricularia grisea in vitro, but they increased the severity level of the rice blaxst disease caused by P. grisea in viro, O2 scavengers were antagonic to probenazole induing resistance against disease in rice, O2 gen - eration was increasing in seedlings treated with probenazole when these seedlings were inoculated with P.grisea. H2O2 and exo-superoxide anion radicals exhibited toxicity to P grisea and rice cells . These results sugest that supoxide anion radicals, raised by probenazole, was invooved in rice diffense re- spontsed against rice blast disease .