红景天多糖对骨髓抑制小鼠表达凋亡相关蛋白的影响

Rhodiola Polysaccharide:Its Effects on Apoptosis Related Protein Expression of Rats with Myelosuppression

目的观察红景天多糖对骨髓抑制小鼠表达凋亡相关蛋白的影响,并探讨其造血调控作用部分机制。方法用免疫组化SABC法分别检测骨髓抑制小鼠骨髓细胞凋亡相关蛋白Caspases-3及Fas/FasL的表达。结果经过射线照射及化疗药物造模后,小鼠骨髓细胞表达Fas/FasL、caspase-3明显增加,用药后各组小鼠骨髓细胞Fas/FasL、caspase-3蛋白表达有不同程度的减少,以高剂量组减少明显(P<0.01)。结论红景天多糖可通过影响骨髓抑制小鼠骨髓细胞死亡受体Fas介导的Fas/FasL-caspase-3凋亡信号通路,减少骨髓细胞凋亡,促进骨髓造血功能的恢复。

Objective： To observe the effects of Rhodiola polysaccharide on apoptosis related protein expression of rats with myelosuppression, and to investigate the mechanism of hematopoietic regulation. Methods： Immunohistochemical SABC method was employed to detect the expression of myelosuppression rats＇ apoptosis related protein Caspases-3 and Fas/FasL. Results： After irradiation and chemotherapy, rats＇bone marrow cells＇expression of Caspases -3 and Fas/FasL increased obviously. After giving the medicine, the expression of Caspases-3 and Fas/FasL of each group decreased in various degrees, and the decrease in the high dosage group was the most obvious （P〈0.01）. Conclusion： Rhodiola poly- saccharide can reduce the apoptosis of bone marrow ceils and promote the recovery of its hemopoietic function by affecting Fas/FasL- caspase-3 apoptosis signal pathway mediated by myelosurression rats＇bone marrow cell death receptor Fas.