摩洛哥脐血橙植株上一种机械传播性病毒的鉴定

DETECTION OF A MECHANICALLY TRANSMISSIBLEE VIRUS IN WASHINGTON SANGUINE (BLOOD)ORANGE TREES IMPORTED FROM MOROCCO

在从摩洛哥引进的脐血橙田间植株的新梢嫩叶上观察到褪绿环斑和污斑，褪绿斑驳，叶背有边缘凹刻的圆形或近圆形环，以及脉明和叶脉环阻等症状。通过向ＭａｄａｍＶｉｎｏｕｓ甜橙、邓肯葡萄柚等柑桔类指示植物的嫁接接种；用钝刀醮取汁液切割指示植物墨西哥来檬和埃特洛香的茎部进行机械传播；以及汁液磨擦接种草本指示植物昆诺藜等方法，鉴定到柑桔环斑病毒（ｃｉｔｒｕｓｒｉｎｇｓｐｏｔｖｉｒｕｓ，ＣＲＳＶ）。嫁接接种的柑桔类指示植物上的症状包括新梢休克、新叶脱落，新叶表现褪绿环斑和污斑以及斑驳，脉明和叶脉环阻等；机械接种的柑桔类指示植物表现新叶的褪绿环斑症状；昆诺藜在接种后４～６ｄ于接种叶上产生褪绿至坏死的局部枯斑，但无系统感染的症状出现。取表现症状的柑桔类指示植物和昆诺藜的叶片作接种源，汁液磨擦接种回接至昆诺藜，再现了同样的症状。

Symptoms of chlorotic rigespots and blotches , chlorotic mottling , ring ething , vein clearing and vein banding were observed in young leaves of Washington Sanguine (blood ) orange trees imported from Morocco in 1965. A mechanically transmissible virus (CRSV ) had been detected by bud-inocu lation to citrus indicatous of Madam Vinous sweet orange and Duncan grapefruit etc. , sap-inoculation from the originally introduced source trees and bud-inoculation citrus seedlings to chenopodium quinoa, as well as by stem knife-cut inoculation to seedlings of Mexican lime and Etrog citron , and back to C. quinoa The graft-inoculated seedlings of Madam Vinous sweet orange , Duncan gragpfruit,Etrog cit- ron , Mexican lime , Goutoucheng and cuttings of Citrus Excelsa all developed different symptoms of shock reaction with leaf shedding in the first flush , and in subsequent flushes , young leaves showed chlorotic spots and blothches , mottling , vein clearing and vein banding. Yong leaves of stem knife-cut inoculated citrus sedings showed chlorotic spots. Chlorotic to necrotic local lesions developed on inoc- ulated C. quinoa leaves 4 - 6 days after inoculation , and with no systematic symptoms in new leaves. Young leaves collected from symptomatic citurs seedlings as inoculum , sap inoculated to C. quinoa, same symptoms were developed.