蜡梅种质资源遗传多样性的ISSR分析

Genetic Diversity of Germplasm Resources of Chimonanthus praecox Based on ISSR Analysis

利用ISSR分子标记技术，对蜡梅种质资源7个野生种群和2个栽培种群的遗传多样性进行了研究。用11条引物，共扩增出124条谱带，其中110条多态带，多态位点占88．70％。用POPEGEN1．31版软件对数据进行分析，结果显示：种群总的Nei’s基因多样性指数为0．2726，Shannon信息多态性指数为0．4117，蜡梅总的遗传多样性水平较高。蜡梅不同种群遗传多样性水平差异较大，种群多态位点百分率在52．94％～90．00％之间，Nei’s基因多样性指数为0．1434～0．3782，Shannon信息多态性指数为0．2322～0．5466。神农架种群（SN）和保康种群（BK）的遗传多样性水平较高。种群间的基因分化系数为0．3536，种群内的遗传变异大于种群间的遗传变异。用NTSYS2．01版软件对样品进行UPGMA聚类分析，结果9个种群并没有按地理距离进行聚类。种群间的地理距离和遗传距离之间没有显著的相关性（r=0．4371，P=0．9213）。

The genetic diversity of 7 wild populations and 2 cultivated populations was analyzed using inter-simple sequence repeat（ISSR） markers. As a result, 124 bands were amplified by 11 informative and reliable primers ,of which 110 were polymorphic loci. As analyzed by the software POPGENE,the genetic diversity of Chimonanthus praecox was high with Nei＇s gene diversity of 0,272 6 and Shannon＇s Information Index of 0.411 7. the genetic diversity difference among populations was high with PPL of 52.94% -90.00% ,Nei＇ s gene diversity of 0. 143 4 -0. 378 2 and Shannon＇ s Information Index of 0. 232 2 - 0.546 6. Genetic differentiation mainly occurred within populations （ GST = 0.353 6 ）. UPGMA cluster analysis based on Nei and Li genetic similarity didn＇ t form 9 clades corresponding to geographic distance. The correlation between genetic distance and geographic distance among the populations was not significant （ r = 0.437 1, P = 0.921 3 ）.