摘要
目的:研究p16基因在K562/A02细胞株的表达情况及其与耐药的关系。方法:用PCR法对慢性髓系白血病细胞株K562及其耐药细胞株K562/A02进行p16基因缺失检测。结果:K562及K562/A02细胞株经PCR扩增后,均未扩出相应条带。结论:K562/A02细胞株与K562细胞株一样,均表现为p16基因缺失。
Objective To discuss the expression of p16 gene in K562/A02 cell line and the relationship between the expression of p16 gene and muhidrug resistance. Method Polymerase chain reaction method was used to detect the expression of p16 gene. Result After PCR, both K562 and K562/A02 cell line had not been found to have amplified stripes. Conclusion Both K562 and K562/A02 cell line show deletion of p16 gene.
出处
《东南大学学报(医学版)》
CAS
2008年第3期151-153,共3页
Journal of Southeast University(Medical Science Edition)
基金
国家自然科学基金资助项目(39970832)