神经干细胞移植治疗大鼠脊髓损伤的活体示踪研究

Neural stem cell transplantation in the treatment of spinal cord injury in rats in vivo traced

目的：探索利用MR技术活体追踪干细胞的可行性及神经干细胞（NSCs）移植对大鼠脊髓损伤（SCI）后功能恢复的影响。方法：66只SD大鼠随机分为假损伤组（A组）、SCI对照组（B组）和细胞移植治疗组（C组）3组，应用已包被多聚左旋赖氨酸（PLL）的SPIO标记NSCs，对标记细胞进行普鲁士蓝染色，分别在细胞移植后第1、2、3、4、5周对各组动物进行BBB评分。细胞移植后1、3、5周行MRI检查。结果：（1）普鲁士蓝染色证实该方法标记NSCs的有效率为100％。（2）细胞移植后1-5周，B、C组动物运动功能均有不同程度恢复，但B组恢复较慢，BBB评分差异有统计学意义（P〈0．05）。（3）1．5TMRI检查见移植处在T2WI序列呈低信号改变，第3周时低信号向损伤区扩大，第5周时可在损伤区见到低信号改变。结论：MR技术可以活体追踪干细胞，NSCs移植治疗SCI有利于大鼠后肢功能的恢复。

Objective To explore the use of magnetic resonance technology tracking stem cells in vivo and the feasibility of neural stem cell transplantation in rat functional recovery after spinal cord injury. Methods 66 SD rats were randomly divided into fake injury group, spinal cord injury group and cell transplantation group, neural stem cells coated with poly-L-lysine （PLL） SPIO marker were stained with Prussian blue and transplanted into rats. 1,2,3,4,5 weeks af- terwards, the animal BBB score was assessed,while MRI was performed in the 1 st,3rd,5th week. Results （ 1 ） Prussian blue stained the neural stem cells in a rate of 100%. （2） Animals in group C and group B recovered their motor functions in a raring degree, but those in the control group did not, BBB score difference was significant （P 〈0.05 ）. （3） T2 WI sequence presented hypointensity in the transplanted area, which expanded into injured areas in 3 weeks. Hypointensity could be seen in the injured area in 5 weeks. Conclusion In vivo MRI technology can track stem cells. Neural stem cell transplantation in the treatment of spinal cord iniury in rats is conducive to the restoration of hind limb function.