摘要
通过瞬间表达快速测定了TA29(烟草花药特异基因)和Zm13(玉米花粉特异基因)启动子在一些植物中的时空表达性,结果表明,TA29启动子在烟草和番茄的花药中具有时空表达性,而Zm13启动子在油菜花粉中无任何表达,这为一些植物构建或转化雄性不育和恢复基因提供了理论依据.此外,还讨论了用基因枪转化的瞬间表达方法。
Temporal and spatial expression activities in some plants of TA29 (tobacco antherspecific gene) and Zm13(maize pollenspecific gene) promoters were measured rapidly by transient expression.The results showed that TA29 pomoter had temporal and spatial expression activities in tobacco and tomato anthers,but Zm13 promoter hadn′t any expression activites in rape pollen,which provided reiable theory evidences for constructing or transforming malesterile and restore genes in some plants.In addition,the feasibility fo measuring antherspecific and pollenspecific genes′ promoters in plants by transient expression of microprojectile bombardment transformation was discussed.
出处
《四川大学学报(自然科学版)》
CAS
CSCD
北大核心
1997年第6期847-851,共5页
Journal of Sichuan University(Natural Science Edition)
基金
国家攀登计划课题
863青年科学基金
中国科学院成都地奥科学基金
关键词
启动子
时空表达
基因
植物
花药
花粉
transient expression,TA29,Zm13,promoter,temporal and spatial expression