微管促解聚与促聚合药物序贯使用对肿瘤细胞的抑制作用

Inhibition Effect of Using Sequential Microtubule Depolymerization Drug and Polymerization Drug on Tumor Cells

目的:探讨细胞微管促解聚与促聚合药物序贯使用对肿瘤细胞的抑制作用。方法:采用四唑盐(MTT)比色法,检测化疗药物紫杉醇和长春瑞滨对肿瘤细胞株MCF-7、SK-OV3、A549的抑瘤效应。设定紫杉醇组、长春瑞滨组、紫杉醇加长春瑞滨组、先紫杉醇4小时后加长春瑞滨组和先长春瑞滨4小时后加紫杉醇组,药物浓度为100%血浆峰值浓度(PPC),50%PPC,25%PPC,12.5%PPC,6.25%PPC,培养72小时测定。结果:对三种肿瘤细胞株MCF-7,SK-OV3,A549,先加长春瑞滨4小时后加紫杉醇组抑瘤效应均明显优于其他各组(P<0.01),先紫杉醇4小时后加长春瑞滨组抑瘤率并不优于其他组(P>0.1)。结论:在联合使用微管解聚或聚合的化疗药物时,先使用促解聚药物,再使用促聚合药物,对肿瘤细胞的抑制有增效作用。

Objective： To investigate the different inhibition effects of different using sequential of microtubule depolymeriza- tlon drug and polymerization drug on tumor cells. Methods： Three tumor cell lines including MCF-7 .SK-OV3.A549 were incubated with paclitaxel（ FiX） and/or vinorelbine （NVB） of different concentrations. The cytotoxicity was examined by MTT test after incubation 72 hours. According to different drugs and different sequences added to 96-well tissue culture plates, 5 groups were divided： PTX group （ Group 1 ）, NVB group （ Group 2）, Frx plus NVB group （ Group 3）, PTX first and plus NVB 4-hours-later group （ Group 4）, NVB first and plus Frx 4-hour-later group （ Group 5）. Drug concentrations were 100% peak plasma concentration （ PPC）, 50% PPC, 25% PPC, 12.5% PPC,and 6. 25% PPC. Results： The inhibition effect on the three tumor ceil lines in Group 5 was stronger than those in the other four groups （ P 〈 0. 01 ）. And the inhibition effect in Group 4 was not stronger than those in Group 1, Group 2 or Group 3 （P 〉 0. 1 ）. Conclusion： Using microtubule depolymerization drug first and then using microtubule pdymerization drug have synergic inhibition effect on tumor cells.